ArticleAIChE journal. American Institute of Chemical Engineers2013
Directed Evolution: Past, Present and Future.
Article in AIChE journal. American Institute of Chemical Engineers, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
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- Protein design drives synthetic biology research of plant natural products.Biodesign research · 2025Review
- Enhancing functional proteins through multimodal inverse folding with ABACUS-T.Nature communications · 2025Article
- Confocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Low-N Protein Activity Optimization with FolDE.ArXiv · 2025Article
- Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporter.Molecular systems biology · 2025Article
- Medical Potential of Insect Symbionts.Insects · 2025Review
- Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis.Chemical reviews · 2024Review
- Strangers in a foreign land: 'Yeastizing' plant enzymes.Microbial biotechnology · 2024Review
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae.Microbial biotechnology · 2024Review
- State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering.SLAS technology · 2024Review
- Article
- Machine learning to predict continuous protein properties from binary cell sorting data and map unseen sequence space.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Improving Protein Expression, Stability, and Function with ProteinMPNN.Journal of the American Chemical Society · 2024Article
- Tuning tRNAs for improved translation.Frontiers in genetics · 2024Review
- Multiple Rounds ofACS synthetic biology · 2023Article
- Computationally guided bioengineering of the active site, substrate access pathway, and water channels of thermostable cytochrome P450, CYP175A1, for catalyzing the alkane hydroxylation reaction.Chemical science · 2023Article
- Bicyclo[3.2.0]carbocyclic Molecules and Redox Biotransformations: The Evolution of Closed-Loop Artificial Linear Biocatalytic Cascades and Related Redox-Neutral Systems.Molecules (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Directed evolution, the laboratory process by which biological entities with desired traits are created through iterative rounds of genetic diversification and library screening or selection, has become one of the most useful and widespread tools in basic and applied biology. From its roots in classical strain engineering and adaptive evolution, modern directed evolution came of age twenty years ago with the demonstration of repeated rounds of PCR-driven random mutagenesis and activity screening to improve protein properties. Since then, numerous techniques have been developed that have enabled the evolution of virtually any protein, pathway, network or entire organism of interest. Here we recount some of the major milestones in the history of directed evolution, highlight the most promising recent developments in the field, and discuss the future challenges and opportunities that lie ahead.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.