ArticleThe Journal of biological chemistry2015
Shu1 is a cell-surface protein involved in iron acquisition from heme in Schizosaccharomyces pombe.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Functional characterization of Hul6 reveals a role in iron-dependent metabolism in Schizosaccharomyces pombe.Archives of microbiology · 2026Article
- The Hemoprotein Hhy1 Promotes Heme-Dependent Catalase Activity of Ctt1.Molecular microbiology · 2026Article
- Dual spatio-functional control of a fission yeast-based bioprocessor upon chemical induction.RSC chemical biology · 2026Article
- The heme-regulated inhibitor kinase Hri1 is activated in response to aminolevulinic acid deficiency in Schizosaccharomyces pombe.PLoS genetics · 2025Article
- Notes from the Underground: Heme Homeostasis inBiomolecules · 2023Review
- Structures and coordination chemistry of transporters involved in manganese and iron homeostasis.Biochemical Society transactions · 2023Review
- Article
- Identification and analysis of iron transporters from the fission yeast Schizosaccharomyces pombe.Archives of microbiology · 2022Article
- Non-mitochondrial aconitase regulates the expression of iron-uptake genes by controlling the RNA turnover process in fission yeast.Journal of microbiology (Seoul, Korea) · 2021Article
- Fungal-Metal Interactions: A Review of Toxicity and Homeostasis.Journal of fungi (Basel, Switzerland) · 2021Review
- Using genetically encoded heme sensors to probe the mechanisms of heme uptake and homeostasis in Candida albicans.Cellular microbiology · 2021Article
- One ring to bring them all and in the darkness bind them: The trafficking of heme without deliverers.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Machinery for fungal heme acquisition.Current genetics · 2020Review
- Article
- Review
- Spore Germination Requires Ferrichrome Biosynthesis and the Siderophore Transporter Str1 inGenetics · 2019Article
- Handling heme: The mechanisms underlying the movement of heme within and between cells.Free radical biology & medicine · 2019Review
- An insight into the iron acquisition and homeostasis in Aureobasidium melanogenum HN6.2 strain through genome mining and transcriptome analysis.Functional & integrative genomics · 2019Article
- The major facilitator transporter Str3 is required for low-affinity heme acquisition inThe Journal of biological chemistry · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron is an essential metal cofactor that is required for many biological processes. Eukaryotic cells have consequently developed different strategies for its acquisition. Until now, Schizosaccharomyces pombe was known to use reductive iron uptake and siderophore-bound iron transport to scavenge iron from the environment. Here, we report the identification of a gene designated shu1(+) that encodes a protein that enables S. pombe to take up extracellular heme for cell growth. When iron levels are low, the transcription of shu1(+) is induced, although its expression is repressed when iron levels rise. The iron-dependent down-regulation of shu1(+) requires the GATA-type transcriptional repressor Fep1, which strongly associates with a proximal promoter region of shu1(+) in vivo in response to iron repletion. HA4-tagged Shu1 localizes to the plasma membrane in cells expressing a functional shu1(+)-HA4 allele. When heme biosynthesis is selectively blocked in mutated S. pombe cells, their ability to acquire exogenous hemin or the fluorescent heme analog zinc mesoporphyrin IX is dependent on the expression of Shu1. Further analysis by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with hemin, with a KD of ∼2.2 μm. Taken together, results reported here revealed that S. pombe possesses an unexpected pathway for heme assimilation, which may also serve as a source of iron for cell growth.
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