ArticleDevelopmental biology2015
A 190 base pair, TGF-β responsive tooth and fin enhancer is required for stickleback Bmp6 expression.
Article in Developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay.Frontiers in immunology · 2026Article
- Article
- Site pleiotropy of a stickleback Bmp6 enhancer.Developmental biology · 2022Article
- Freshwater Colonization, Adaptation, and Genomic Divergence in Threespine Stickleback.Integrative and comparative biology · 2022Article
- Evolved Bmp6 enhancer alleles drive spatial shifts in gene expression during tooth development in sticklebacks.Genetics · 2021Article
- Article
- Efficient CRISPR-Cas9 editing of major evolutionary loci in sticklebacks.Evolutionary ecology research · 2019Article
- An intronic enhancer of Bmp6 underlies evolved tooth gain in sticklebacks.PLoS genetics · 2018Article
- Genetic Dissection of a Supergene ImplicatesGenetics · 2018Article
- Article
- Sequence-Based Mapping and Genome Editing Reveal Mutations in SticklebackG3 (Bethesda, Md.) · 2017Article
- The genetic and molecular architecture of phenotypic diversity in sticklebacks.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Early development and replacement of the stickleback dentition.Journal of morphology · 2016Article
- Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks.Journal of visualized experiments : JoVE · 2016Article
- Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton.Journal of visualized experiments : JoVE · 2016Article
- Genomics of Rapid Incipient Speciation in Sympatric Threespine Stickleback.PLoS genetics · 2016Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The ligands of the Bone Morphogenetic Protein (BMP) family of developmental signaling molecules are often under the control of complex cis-regulatory modules and play diverse roles in vertebrate development and evolution. Here, we investigated the cis-regulatory control of stickleback Bmp6. We identified a 190bp enhancer ~2.5 kilobases 5' of the Bmp6 gene that recapitulates expression in developing teeth and fins, with a core 72bp sequence that is sufficient for both domains. By testing orthologous enhancers with varying degrees of sequence conservation from outgroup teleosts in transgenic reporter gene assays in sticklebacks and zebrafish, we found that the function of this regulatory element appears to have been conserved for over 250 million years of teleost evolution. We show that a predicted binding site for the TGFβ effector Smad3 in this enhancer is required for enhancer function and that pharmacological inhibition of TGFβ signaling abolishes enhancer activity and severely reduces endogenous Bmp6 expression. Finally, we used TALENs to disrupt the enhancer in vivo and find that Bmp6 expression is dramatically reduced in teeth and fins, suggesting this enhancer is necessary for expression of the Bmp6 locus. This work identifies a relatively short regulatory sequence that is required for expression in multiple tissues and, combined with previous work, suggests that shared regulatory networks control limb and tooth development.
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