Evidence map›Paper›PMID 25732513›Full record

ArticleScientific reports2015

MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro.

Ruizhi Feng, Qing Sang, Yan Zhu, Wei Fu, Miao Liu, Yan Xu, Huijuan Shi, Yao Xu, Ronggui Qu, Renjie Chai and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed, 2 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 2 syntheses or guidelines pooled it, 102 citations in OpenAlex.

  1. Pooled it
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  10. Article
  11. Review
  12. Human embryo implantation.Development (Cambridge, England) · 2023
    Review
  13. Mechanisms of ovarian aging in women: a review.Journal of ovarian research · 2023
    Review
  14. Article
  15. Observational
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 2 countries.

Ruizhi Feng1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Qing Sang1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Yan ZhuGuangdong No.2 provincial people's hospital, Guangzhou, China.
Wei FuShanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Miao LiuShanghai Institute of Planned Parenthood Research, Shanghai, China.
Yan XuShanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Huijuan ShiShanghai Institute of Planned Parenthood Research, Shanghai, China.
Yao Xu1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Ronggui Qu1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Renjie ChaiKey Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
Ruijin ShaoDepartment of Physiology/Endocrinology, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.
Li JinState Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.
Lin He1] Institutes of Biomedical Sciences, Fudan University, Shanghai, China [2] Bio-X Center, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China.
Xiaoxi SunShanghai Ji Ai Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Lei Wang1] State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Fudan University · CNShanghai Institute of Planned Parenthood Research · CNState Key Laboratory of Genetic EngineeringGuangdong Provincial People's Hospital · CNSoutheast University · CNUniversity of Gothenburg · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous work from our laboratory demonstrated the existence of miRNAs in human follicular fluid. In the current study, we have sought to identify miRNAs that might affect oocyte/embryo quality in patients undergoing intracytoplasmic sperm injection and to investigate their roles in in vitro fertilization outcomes in mouse oocytes. 53 samples were classified as Group 1 (high quality) if the day-3 embryos had seven and more cells or as Group 2 (low quality) if the embryos had six and fewer cells. TaqMan Human microRNAs cards and qRT-PCR were performed to verify differently expressed miRNAs. The function of the corresponding miRNA was investigated in mouse oocytes by injecting them with miRNA-inhibitor oligonucleotides. We found that hsa-miR-320a and hsa-miR-197 had significantly higher expression levels in the Group 1 follicular fluids than in Group 2 (p = 0.0073 and p = 0.008, respectively). Knockdown of mmu-miR-320 in mouse oocytes strongly decreased the proportions of MII oocytes that developed into two-cell and blastocyst stage embryos (p = 0.0048 and p = 0.0069, respectively). Wnt signaling pathway components had abnormal expression level in miR-320 inhibitor-injected oocytes. This study provides the first evidence that miRNAs in human follicular fluid are indicative of and can influence embryo quality.

Indexed as

Fertilization in VitroFollicular FluidAnimalsBlastocystEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Knockdown TechniquesHumansMiceMicroRNAsOocytesRNA InterferenceWnt Signaling PathwayMicroRNAsMIRN320 microRNA, human

Identifiers

PMID25732513
PMCPMC4346788
OpenAlexW2000787189

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.