ArticleToxicology and applied pharmacology2015
Developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin alters DNA methyltransferase (dnmt) expression in zebrafish (Danio rerio).
Article in Toxicology and applied pharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- Preliminary insights into methylation patterns in Agent Orange exposed thyroid cancers: a pilot study.Environmental pollution (Barking, Essex : 1987) · 2026Article
- High Doses of Norfloxacin Nicotinate Induce Apoptosis, Developmental Neurotoxicity, and Aberrant DNA Methylation in Zebrafish (Animals : an open access journal from MDPI · 2025Article
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- Fine particulate matter‑induced cardiac developmental toxicity (Review).Experimental and therapeutic medicine · 2025Review
- DNA Methylation Profiling of Ovarian Tissue of Climbing Perch (Anabas testudienus) in Response to Monocrotophos Exposure.Marine biotechnology (New York, N.Y.) · 2023Article
- Exposure to 3,3',4,4',5-Pentachlorobiphenyl (PCB126) Causes Widespread DNA Hypomethylation in Adult Zebrafish Testis.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- mRNA Expressions of Methylation Related Enzymes and Duration of Thermal Conditioning in Chicks.The journal of poultry science · 2022Article
- Sources of variation of DNA methylation in rainbow trout: combined effects of temperature and genetic background.Epigenetics · 2021Article
- Upregulated PPARG2 facilitates interaction with demethylated AKAP12 gene promoter and suppresses proliferation in prostate cancer.Cell death & disease · 2021Article
- A Review of the Functional Roles of the Zebrafish Aryl Hydrocarbon Receptors.Toxicological sciences : an official journal of the Society of Toxicology · 2020Review
- Dioxin Disrupts Dynamic DNA Methylation Patterns in Genes That Govern Cardiomyocyte Maturation.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- DuplicatedGenes · 2020Article
- Epigenetic Regulations of AhR in the Aspect of Immunomodulation.International journal of molecular sciences · 2020Review
- Evolutionary history of DNA methylation related genes in chordates: new insights from multiple whole genome duplications.Scientific reports · 2020Article
- TCDD-induced multi- and transgenerational changes in the methylome of male zebrafish gonads.Environmental epigenetics · 2020Article
- TCDD Toxicity Mediated by Epigenetic Mechanisms.International journal of molecular sciences · 2018Review
- Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish.Scientific reports · 2018Article
- Epigenetic profiling to environmental stressors in model and non-model organisms: Ecotoxicology perspective.Environmental health and toxicology · 2018Article
- Role of DNA methylation in altered gene expression patterns in adult zebrafish (Environmental epigenetics · 2018Article
- Developmental Dioxin Exposure Alters the Methylome of Adult Male Zebrafish Gonads.Frontiers in genetics · 2018Article
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
DNA methylation is one of the most important epigenetic modifications involved in the regulation of gene expression. The DNA methylation reaction is catalyzed by DNA methyltransferases (DNMTs). Recent studies have demonstrated that toxicants can affect normal development by altering DNA methylation patterns, but the mechanisms of action are poorly understood. Hence, we tested the hypothesis that developmental exposure to TCDD affects dnmt gene expression patterns. Zebrafish embryos were exposed to 5nM TCDD for 1h from 4 to 5h post-fertilization (hpf) and sampled at 12, 24, 48, 72, and 96 hpf to determine dnmt gene expression and DNA methylation patterns. We performed a detailed analysis of zebrafish dnmt gene expression during development and in adult tissues. Our results demonstrate that dnmt3b genes are highly expressed in early stages of development, and dnmt3a genes are more abundant in later stages. TCDD exposure upregulated dnmt1 and dnmt3b2 expression, whereas dnmt3a1, 3b1, and 3b4 are downregulated following exposure. We did not observe any TCDD-induced differences in global methylation or hydroxymethylation levels, but the promoter methylation of aryl hydrocarbon receptor (AHR) target genes was altered. In TCDD-exposed embryos, AHR repressor a (ahrra) and c-fos promoters were differentially methylated. To characterize the TCDD effects on DNMTs, we cloned the dnmt promoters with xenobiotic response elements and conducted AHR transactivation assays using a luciferase reporter system. Our results suggest that ahr2 can regulate dnmt3a1, dnmt3a2, and dnmt3b2 expression. Overall, we demonstrate that developmental exposure to TCDD alters dnmt expression and DNA methylation patterns.
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