ArticleCell host & microbe2015
A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite.
Article in Cell host & microbe, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed.
- TKL3 regulates blood-stage fitness, male gamete fertility, and transmission-stage development in Plasmodium berghei.PLoS pathogens · 2026Article
- Bi-directional highways and super-seeder tissues underpin parasite dissemination in experimental Leishmania donovani infection.Nature communications · 2026Article
- Article
- Validated antimalarial drug target discovery using genome-scale metabolic modeling.Antimicrobial agents and chemotherapy · 2025Article
- Plasmodium actin-like proteins are essential for DNA segregation during male gametogenesis and malaria transmission.PLoS pathogens · 2025Article
- The ribosomal RNA transcription landscapes of Plasmodium falciparum and related apicomplexan parasites.Nucleic acids research · 2025Review
- PfPPM2 signalling regulates asexual division and sexual conversion of human malaria parasite Plasmodium falciparum.Nature communications · 2025Article
- A Plasmodium LARC GAP provides preerythrocytic, stage and species transcending protection in mice.NPJ vaccines · 2025Article
- A CRISPR homing screen finds a chloroquine resistance transporter-like protein of the Plasmodium oocyst essential for mosquito transmission of malaria.Nature communications · 2025Article
- Microtubule inner proteins ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Supersaturation mutagenesis reveals adaptive rewiring of essential genes among malaria parasites.Science (New York, N.Y.) · 2025Article
- YAT2150 is irresistible in Plasmodium falciparum and active against Plasmodium vivax and Leishmania clinical isolates.Scientific reports · 2025Article
- A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei.Nucleic acids research · 2025Article
- Nuclear pore complexes undergo Nup221 exchange during blood-stage asexual replication ofmSphere · 2024Article
- Experimental vaccination by single dose sporozoite injection of blood-stage attenuated malaria parasites.EMBO molecular medicine · 2024Article
- The multifaceted roles of Myb domain-containing proteins in apicomplexan parasites.Current opinion in microbiology · 2023Review
- DNA-binding protein PfAP2-P regulates parasite pathogenesis during malaria parasite blood stages.Nature microbiology · 2023Article
- Regulation of Atg8 membrane deconjugation by cysteine proteases in the malaria parasite Plasmodium berghei.Cellular and molecular life sciences : CMLS · 2023Article
- IntracellularProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Identification of genes required for Plasmodium gametocyte-to-sporozoite development in the mosquito vector.Cell host & microbe · 2023Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
The genome-wide identification of gene functions in malaria parasites is hampered by a lack of reverse genetic screening methods. We present a large-scale resource of barcoded vectors with long homology arms for effective modification of the Plasmodium berghei genome. Cotransfecting dozens of vectors into the haploid blood stages creates complex pools of barcoded mutants, whose competitive fitness can be measured during infection of a single mouse using barcode sequencing (barseq). To validate the utility of this resource, we rescreen the P. berghei kinome, using published kinome screens for comparison. We find that several protein kinases function redundantly in asexual blood stages and confirm the targetability of kinases cdpk1, gsk3, tkl3, and PBANKA_082960 by genotyping cloned mutants. Thus, parallel phenotyping of barcoded mutants unlocks the power of reverse genetic screening for a malaria parasite and will enable the systematic identification of genes essential for in vivo parasite growth and transmission.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.