A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium.

Jie Shen, Changzu Cai, Zhilong Yu, Yuhong Pang, Ying Zhou, Lili Qian, Wensheng Wei, Yanyi Huang

Abstract read

In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

The Growing Importance of Three-Dimensional Models and Microphysiological Systems in the Assessment of Mycotoxin Toxicity.Toxins · 2023
Review
Microfluidic-Based Single-Cell Study: Current Status and Future Perspective.Molecules (Basel, Switzerland) · 2018
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jie Shen1] Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China [2] College of Engineering, Peking University, Beijing, 100871, China [3] School of Life Sciences, Peking University, Beijing, 100871, China.

Changzu CaiSchool of Life Sciences, Peking University, Beijing, 100871, China.

Zhilong Yu1] Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China [2] College of Engineering, Peking University, Beijing, 100871, China.

Yuhong Pang1] Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China [2] School of Life Sciences, Peking University, Beijing, 100871, China.

Ying Zhou1] Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China [2] College of Engineering, Peking University, Beijing, 100871, China.

Lili QianSchool of Life Sciences, Peking University, Beijing, 100871, China.

Wensheng WeiSchool of Life Sciences, Peking University, Beijing, 100871, China.

Yanyi Huang1] Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, 100871, China [2] College of Engineering, Peking University, Beijing, 100871, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It is technically challenging to investigate the function of secreted protein in real time by supply of conditioned medium that contains secreted protein of interest. The internalization of anthrax toxin is facilitated by a secreted protein Dickkopf-1 (DKK1) and its receptor, and eventually leads to cell lethality. To monitor the dynamic interplay between these components in live cells, we use an integrated microfluidic device to perform the cell viability assays with real-time controlled culture microenvironment in parallel. Conditioned medium, which contains the secreted proteins from specific cell lines, can be continuously pumped towards the cells that exposed to toxin. The exogenous DKK1 secreted from distant cells is able to rescue the sensitivity to toxin for those DKK1-knocked-down cells. This high-throughput assay allows us to precisely quantify the dynamic interaction between key components that cause cell death, and provide independent evidence of the function of DKK1 in the complex process of anthrax toxin internalization.

Indexed as

AnimalsAntigens, BacterialBacterial ToxinsCell Culture TechniquesCell LineCell SurvivalCulture Media, ConditionedGene ExpressionGene Knockdown TechniquesHigh-Throughput Screening AssaysHumansIntercellular Signaling Peptides and ProteinsMiceMicrofluidicsRNA Interferenceanthrax toxinAntigens, BacterialBacterial ToxinsCulture Media, ConditionedDkk1 protein, mouseIntercellular Signaling Peptides and Proteins

Identifiers

PMID25731605

DOI10.1038/srep08651

PMCPMC4346806

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.