ArticleNature structural & molecular biology2015
Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex.
Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNA.Cell reports · 2022Article
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- Active retrotransposons help maintain pericentromeric heterochromatin required for faithful cell division.Genome research · 2020Article
- Review
- A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.eLife · 2020Article
- Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.Molecular cell · 2020Article
- Bacterium-Mediated RNA Interference: Potential Application in Plant Protection.Plants (Basel, Switzerland) · 2019Review
- Argonaute bypasses cellular obstacles without hindrance during target search.Nature communications · 2019Article
- Chromatin-associated RNAs as facilitators of functional genomic interactions.Nature reviews. Genetics · 2019Review
- Suppressors of Meiotic Silencing by Unpaired DNA.Non-coding RNA · 2019Article
- Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.Communications biology · 2019Article
- RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.Epigenetics & chromatin · 2018Article
- DICER- and MMSET-catalyzed H4K20me2 recruits the nucleotide excision repair factor XPA to DNA damage sites.The Journal of cell biology · 2018Article
- Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription.Nature · 2017Article
- The RNAi Inheritance Machinery ofGenetics · 2017Article
- DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair.Nucleic acids research · 2017Article
- New methods in the diagnosis of cancer and gene therapy of cancer based on nanoparticles.Cancer gene therapy · 2017Review
- Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.Molecular cell · 2016Article
- Beyond Tethering and the LEM domain: MSCellaneous functions of the inner nuclear membrane Lem2.Nucleus (Austin, Tex.) · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Argonautes and their small-RNA cofactors form the core effectors of ancient and diverse gene-silencing mechanisms whose roles include regulation of gene expression and defense against foreign genetic elements. Although Argonautes generally act within multisubunit complexes, what governs their assembly into these machineries is not well defined. Here, we show that loading of small RNAs onto Argonaute is a checkpoint for Argonaute's association with conserved GW-protein components of silencing complexes. We demonstrate that the Argonaute small interfering RNA chaperone (ARC) complex mediates loading of small RNAs onto Ago1 in Schizosaccharomyces pombe and that deletion of its subunits, or mutations in Ago1 that prevent small-RNA loading, abolish the assembly of the GW protein-containing RNA-induced transcriptional silencing (RITS) complex. Our studies uncover a mechanism that ensures that Argonaute loading precedes RITS assembly and thereby averts the formation of inert and potentially deleterious complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.