ArticleFrontiers in neurology2015
Effects of blast overpressure on neurons and glial cells in rat organotypic hippocampal slice cultures.
Article in Frontiers in neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Neuroglial Response to High-Amplitude, Short-Duration Pressure Transients in Monoculture.Cell biochemistry and function · 2026Article
- Experimental wound ballistic study to understand biomechanical differences in gunshot wounds from various bullets and firearms: implications for clinical care and forensic analysis.Forensic science, medicine, and pathology · 2026Article
- Potential of ex vivo organotypic slice cultures in neuro-oncology.Neuro-oncology · 2025Review
- Blast-Overpressure Induced Modulation of PARP-SIRT-NRF2 Axis in Stress Signaling of Astrocytes and Microglia.Immunity, inflammation and disease · 2025Article
- Partial Depletion of Microglia Attenuates Long-Term Potentiation Deficits following Repeated Blast Traumatic Brain Injury in Organotypic Hippocampal Slice Cultures.Journal of neurotrauma · 2023Article
- Recent progress in translational engineered in vitro models of the central nervous system.Brain : a journal of neurology · 2020Review
- Calycosin Preserves BDNF/TrkB Signaling and Reduces Post-Stroke Neurological Injury after Cerebral Ischemia by Reducing Accumulation of Hypertrophic and TNF-α-Containing Microglia in Rats.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2020Article
- Photobiomodulation (660 nm) therapy reduces oxidative stress and induces BDNF expression in the hippocampus.Scientific reports · 2019Article
- Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT).Scientific reports · 2019Article
- Exposure to mild blast forces induces neuropathological effects, neurophysiological deficits and biochemical changes.Molecular brain · 2018Article
- Xenon Protects against Blast-Induced Traumatic Brain Injury in an In Vitro Model.Journal of neurotrauma · 2018Article
- On the Accurate Determination of Shock Wave Time-Pressure Profile in the Experimental Models of Blast-Induced Neurotrauma.Frontiers in neurology · 2018Article
- Evaluating Primary Blast Effects In Vitro.Journal of visualized experiments : JoVE · 2017Article
- Acute death of astrocytes in blast-exposed rat organotypic hippocampal slice cultures.PloS one · 2017Article
- Blast Scaling Parameters: Transitioning from Lung to Skull Base Metrics.Journal of surgery and emergency medicine · 2017Article
- Behavioral Outcomes Differ between Rotational Acceleration and Blast Mechanisms of Mild Traumatic Brain Injury.Frontiers in neurology · 2016Article
- A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model.Basic and clinical neuroscienceArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to recent involvement in military conflicts, and an increase in the use of explosives, there has been an escalation in the incidence of blast-induced traumatic brain injury (bTBI) among US military personnel. Having a better understanding of the cellular and molecular cascade of events in bTBI is prerequisite for the development of an effective therapy that currently is unavailable. The present study utilized organotypic hippocampal slice cultures (OHCs) exposed to blast overpressures of 150 kPa (low) and 280 kPa (high) as an in vitro bTBI model. Using this model, we further characterized the cellular effects of the blast injury. Blast-evoked cell death was visualized by a propidium iodide (PI) uptake assay as early as 2 h post-injury. Quantification of PI staining in the cornu Ammonis 1 and 3 (CA1 and CA3) and the dentate gyrus regions of the hippocampus at 2, 24, 48, and 72 h following blast exposure revealed significant time dependent effects. OHCs exposed to 150 kPa demonstrated a slow increase in cell death plateauing between 24 and 48 h, while OHCs from the high-blast group exhibited a rapid increase in cell death already at 2 h, peaking at ~24 h post-injury. Measurements of lactate dehydrogenase release into the culture medium also revealed a significant increase in cell lysis in both low- and high-blast groups compared to sham controls. OHCs were fixed at 72 h post-injury and immunostained for markers against neurons, astrocytes, and microglia. Labeling OHCs with PI, neuronal, and glial markers revealed that the blast-evoked extensive neuronal death and to a lesser extent loss of glial cells. Furthermore, our data demonstrated activation of astrocytes and microglial cells in low- and high-blasted OHCs, which reached a statistically significant difference in the high-blast group. These data confirmed that our in vitro bTBI model is a useful tool for studying cellular and molecular changes after blast exposure.
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