ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2015
Whole transcriptome expression profiling of mouse limb tendon development by using RNA-seq.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Understanding Tendons: Lessons from Transgenic Mouse Models.Stem cells and development · 2018Pooled it
- Signaling and transcriptional networks governing late synovial joint development.Developmental biology · 2026Article
- Article
- Gene regulatory programs underlying diversification of facial ligaments and tendons in zebrafish.Development (Cambridge, England) · 2026Article
- The roles of TGF-β, Wnt, and MAPK signaling pathways in joint lineage specification in vitro and ex vivo.Stem cell reports · 2025Article
- Dynamic transcriptional and epigenetic changes define postnatal tendon growth.PLoS genetics · 2025Article
- Article
- A conserved transcription factor regulatory program promotes tendon fate.Developmental cell · 2024Article
- Article
- Nonsurgical treatment reduces tendon inflammation and elevates tendon markers in early healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Research perspectives-Pipelines to human tendon transcriptomics.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Review
- Coordinate roles for collagen VI and biglycan in regulating tendon collagen fibril structure and function.Matrix biology plus · 2022Article
- Tendon and multiomics: advantages, advances, and opportunities.NPJ Regenerative medicine · 2021Review
- Transcriptional profiling of mESC-derived tendon and fibrocartilage cell fate switch.Nature communications · 2021Article
- Development and maintenance of tendons and ligaments.Development (Cambridge, England) · 2021Review
- Voltage-Gated Calcium Channels in Nonexcitable Tissues.Annual review of physiology · 2021Review
- The regulatory roles and mechanisms of the transcription factor FOXF2 in human diseases.PeerJ · 2021Article
- The Scleraxis Transcription Factor Directly Regulates Multiple Distinct Molecular and Cellular Processes During Early Tendon Cell Differentiation.Frontiers in cell and developmental biology · 2021Article
- Transcriptomic and Genetic Analyses Identify the Krüppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development.Frontiers in cell and developmental biology · 2021Article
- A single cell transcriptional atlas of early synovial joint development.Development (Cambridge, England) · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Tendons are fibrous connective tissues that transmit force between muscle and bone. Whereas the molecular and cellular mechanisms of bone and muscle development have been well studied, that of tendon development is poorly understood. Using the Scx-GFP transgenic mice, we isolated GFP(+) cells from the developing mouse limbs at E11.5, E13.5, and E15.5, respectively, and carried out whole transcriptome RNA-seq analysis. Comparing the gene expression profiles of GFP(+) and GFP(-) cells in the E13.5 limb isolated over 1,500 genes that exhibited enrichment of mRNA expression by at least 1.5-fold in the GFP(+) cells. Of these, 778 genes showed expression up-regulated by more than 1.5-fold from E11.5 to E13.5 and 516 genes showed expression up-regulated by more than 1.5-fold from E13.5 to E15.5 in the GFP(+) cell population. Interestingly, over 30 genes encoding transcription factors are among the early-activated genes in the GFP(+) cells. Whole mount and section in situ hybridization analyses showed that many of these transcription factor genes have distinct patterns of expression during limb development and identified Foxf2 expression as a specific marker for differentiated dorsal limb tendon cells. Together, these data provide a valuable resource for further investigation of the molecular mechanisms regulating tendon development.
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