ArticleThe Journal of comparative neurology2015
Potential and limitations of X-Ray micro-computed tomography in arthropod neuroanatomy: a methodological and comparative survey.
Article in The Journal of comparative neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed.
- Comparative Neuroanatomy of Hydrothermal Vent Shrimps: Ecological Differentiation Among Four Alvinocaridid Species.The Journal of comparative neurology · 2026Article
- High-throughput phenomics of global ant biodiversity.Nature methods · 2026Article
- The green lacewing venom system and the complex mechanisms underlying its evolution.Molecular biology and evolution · 2026Article
- Sensory Enrichment and Deprivation During Development: Limited Effects on the Volumes of CNS Neuropils in Two Spiders With Different Ecology.The Journal of comparative neurology · 2025Article
- Article
- Specimen preparation for X-ray micro-computed tomography of forest pests.Applied microscopy · 2025Review
- In situ isotropic 3D imaging of vasculature perfusion specimens using x-ray microscopic dual-energy CT.Journal of microscopy · 2025Article
- Comparing microCT Staining and Scanning Methodology for Brain Studies in Various Sizes of Spiders.The Journal of comparative neurology · 2025Article
- The SmARTR pipeline: A modular workflow for the cinematic rendering of 3D scientific imaging data.iScience · 2024Article
- Revisiting the scorpion central nervous system using microCT.Scientific reports · 2024Article
- Small Brains: Body Shape Constrains Tissue Allocation to the Central Nervous System in Ant-Mimicking Spiders.The Journal of comparative neurology · 2024Article
- Explosive regeneration and anamorphic development of legs in the house centipede Scutigera coleoptrata.Frontiers in zoology · 2024Article
- Material composition and mechanical properties of the venom-injecting forcipules in centipedes.Frontiers in zoology · 2024Article
- The venom and telopodal defence systems of the centipede Lithobius forficatus are functionally convergent serial homologues.BMC biology · 2024Article
- Anatomical changes of Tenebrio molitor and Tribolium castaneum during complete metamorphosis.Cell and tissue research · 2024Article
- Article
- Bioimaging marine crustacean brain: quantitative comparison of micro-CT preparations in an Alpheid snapping shrimp.Frontiers in neuroscience · 2024Article
- Natural variability in bee brain size and symmetry revealed by micro-CT imaging and deep learning.PLoS computational biology · 2023Article
- Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging.PNAS nexus · 2023Article
- Micro-CT visualization of the CNS: Performance of different contrast-enhancing techniques for documenting the spider brain.The Journal of comparative neurology · 2022Article
5 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Classical histology or immunohistochemistry combined with fluorescence or confocal laser scanning microscopy are common techniques in arthropod neuroanatomy, and these methods often require time-consuming and difficult dissections and sample preparations. Moreover, these methods are prone to artifacts due to compression and distortion of tissues, which often result in information loss and especially affect the spatial relationships of the examined parts of the nervous system in their natural anatomical context. Noninvasive approaches such as X-ray micro-computed tomography (micro-CT) can overcome such limitations and have been shown to be a valuable tool for understanding and visualizing internal anatomy and structural complexity. Nevertheless, knowledge about the potential of this method for analyzing the anatomy and organization of nervous systems, especially of taxa with smaller body size (e.g., many arthropods), is limited. This study set out to analyze the brains of selected arthropods with micro-CT, and to compare these results with available histological and immunohistochemical data. Specifically, we explored the influence of different sample preparation procedures. Our study shows that micro-CT is highly suitable for analyzing arthropod neuroarchitecture in situ and allows specific neuropils to be distinguished within the brain to extract quantitative data such as neuropil volumes. Moreover, data acquisition is considerably faster compared with many classical histological techniques. Thus, we conclude that micro-CT is highly suitable for targeting neuroanatomy, as it reduces the risk of artifacts and is faster than classical techniques.
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