ArticleScientific reports2015
GSK3 inhibitors CHIR99021 and 6-bromoindirubin-3'-oxime inhibit microRNA maturation in mouse embryonic stem cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Electrospun CHIR99021-loaded cellulose acetate scaffolds stimulate osteogenic differentiation and mineralization in vitro.Journal of materials science. Materials in medicine · 2026Article
- Novel feeder-cell-free 3C system promotes the transition from primed to formative pluripotency, self-renewal, and germline differentiation in rabbit embryonic stem cellsFrontiers in cell and developmental biology · 2026Article
- WNT-mediating TCF/LEF transcription factor gene expression in early human pluripotency and cell lineages differs from the rodent paradigm.Journal of cell science · 2025Article
- RNF2 regulates Wnt/ß-catenin signaling via TCF7L1 destabilization.Scientific reports · 2023Article
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- GSK3β Interacts With CRMP2 and Notch1 and Controls T-Cell Motility.Frontiers in immunology · 2021Article
- GSK3β: A Master Player in Depressive Disorder Pathogenesis and Treatment Responsiveness.Cells · 2020Review
- Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice.Animal cells and systems · 2020Article
- 6-Bromoindirubin-3'-oxime intercepts GSK3 signaling to promote and enhance skeletal muscle differentiation affecting miR-206 expression in mice.Scientific reports · 2019Article
- CDX2 inhibits the proliferation and tumor formation of colon cancer cells by suppressing Wnt/β-catenin signaling via transactivation of GSK-3β and Axin2 expression.Cell death & disease · 2019Article
- Assaying Chromatin Accessibility Using ATAC-Seq in Invertebrate Chordate Embryos.Frontiers in cell and developmental biology · 2019Article
- Noncoding RNAs in the Regulation of Pluripotency and Reprogramming.Stem cell reviews and reports · 2018Review
- β-TrCP1 Is a Vacillatory Regulator of Wnt Signaling.Cell chemical biology · 2017Article
- A novel role for GSK3β as a modulator of Drosha microprocessor activity and MicroRNA biogenesis.Nucleic acids research · 2017Article
- CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells.Oncotarget · 2017Article
- 6BIO Enhances Oligonucleotide Activity in Cells: A Potential Combinatorial Anti-androgen Receptor Therapy in Prostate Cancer Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264.7) Resistance to Mycobacterium tuberculosis H37Ra.Scientific reports · 2016Article
- Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901.Stem cells international · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wnt/β-catenin signalling plays a prominent role in maintaining self-renewal and pluripotency of mouse embryonic stem cells (mESCs). microRNAs (miRNAs) have critical roles in maintaining pluripotency and directing reprogramming. To investigate the effect of GSK3 inhibitors on miRNA expression, we analysed the miRNA expression profile of J1 mESCs in the absence or presence of CHIR99021 (CHIR) or 6-bromoindirubin-3'-oxime (BIO) by small RNA deep-sequencing. The results demonstrate that CHIR and BIO decrease mature miRNAs of most miRNA species, 90.4% and 98.1% of the differentially expressed miRNAs in BIO and CHIR treated cells were downregulated respectively. CHIR and BIO treatment leads to a slight upregulation of the primary transcripts of the miR-302-367 cluster and miR-181 family of miRNAs, these miRNAs are activated by Wnt/β-catenin signalling. However, the precursor and mature form of the miR-302-367 cluster and miR-181 family of miRNAs are downregulated by CHIR, suggesting CHIR inhibits maturation of primary miRNA. Western blot analysis shows that BIO and CHIR treatment leads to a reduction of the RNase III enzyme Drosha in the nucleus. These data suggest that BIO and CHIR inhibit miRNA maturation by disturbing nuclear localisation of Drosha. Results also show that BIO and CHIR induce miR-211 expression in J1 mESCs.
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