ArticleEnvironmental microbiology2015
Regulation of plasmid-encoded isoprene metabolism in Rhodococcus, a representative of an important link in the global isoprene cycle.
Article in Environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Catabolism of diethyl ether as a sole carbon and energy source by Mycolicibacterium sp. ELW1.Biodegradation · 2026Article
- A Diffusion-Driven CH4-O2 Boundary Structures Methane Oxidation and Carbon Transformation in Upland Soils.Environmental science & technology · 2026Article
- Aerobic catabolism of isobutylene byApplied and environmental microbiology · 2026Article
- Structural and evolutionary insights into the isoprene monooxygenases.FEMS microbiology ecology · 2026Article
- Decoding plant volatile stress signals across scales: from molecular responses to ecosystem dynamics.Frontiers in plant science · 2026Review
- Isoprene Production by Sphagnum Moss Is Balanced by Microbial Uptake, as Revealed by Selective Inhibitors.Environmental microbiology · 2025Article
- Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway.Current microbiology · 2025Article
- Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases.Environmental microbiology · 2025Review
- Biodegradation of isoprene by soil Actinomycetota from coffee-tea integrated plantations in a tropical evergreen forest.Current research in microbial sciences · 2025Article
- Whole-cell studies of substrate and inhibitor specificity of isoprene monooxygenase and related enzymes.Environmental microbiology reports · 2023Article
- AraC-Based Biosensor for the Detection of Isoprene inACS omega · 2023Article
- Analysis of Essential Isoprene Metabolic Pathway Proteins inApplied and environmental microbiology · 2023Article
- 'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.Environmental microbiology · 2022Article
- Purification and Characterization of the Isoprene Monooxygenase fromApplied and environmental microbiology · 2022Article
- Genome Characterisation of an Isoprene-DegradingBiology · 2022Article
- Characterization of the GlutathioneMicrobiology spectrum · 2021Article
- Isoprene-degrading bacteria associated with the phyllosphere of Salix fragilis, a high isoprene-emitting willow of the Northern Hemisphere.Environmental microbiome · 2021Article
- Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Microbial metabolism of isoprene: a much-neglected climate-active gas.Microbiology (Reading, England) · 2020Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Emissions of biogenic volatile organic compounds (VOCs) form an important part of the global carbon cycle, comprising a significant proportion of net ecosystem productivity. They impact atmospheric chemistry and contribute directly and indirectly to greenhouse gases. Isoprene, emitted largely from plants, comprises one third of total VOCs, yet in contrast to methane, which is released in similar quantities, we know little of its biodegradation. Here, we report the genome of an isoprene degrading isolate, Rhodococcus sp. AD45, and, using mutagenesis shows that a plasmid-encoded soluble di-iron centre isoprene monooxygenase (IsoMO) is essential for isoprene metabolism. Using RNA sequencing (RNAseq) to analyse cells exposed to isoprene or epoxyisoprene in a substrate-switch time-course experiment, we show that transcripts from 22 contiguous genes, including those encoding IsoMO, were highly upregulated, becoming among the most abundant in the cell and comprising over 25% of the entire transcriptome. Analysis of gene transcription in the wild type and an IsoMO-disrupted mutant strain showed that epoxyisoprene, or a subsequent product of isoprene metabolism, rather than isoprene itself, was the inducing molecule. We provide a foundation of molecular data for future research on the environmental biological consumption of this important, climate-active compound.
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