ArticleJournal of biophotonics2015
Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres.
Article in Journal of biophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Correlating NAD(P)H lifetime shifts to tamoxifen resistance in breast cancer cells: A metabolic screening study with time-resolved flow cytometry.Journal of innovative optical health sciences · 2025Article
- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dynamic fluorescence lifetime sensing with CMOS single-photon avalanche diode arrays and deep learning processors.Biomedical optics express · 2021Article
- Toward the Development of an On-Chip Acoustic Focusing Fluorescence Lifetime Flow Cytometer.Frontiers in physics · 2021Article
- A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues.Frontiers in physics · 2021Article
- Evaluation of Caspase-3 Activity During Apoptosis with Fluorescence Lifetime-Based Cytometry Measurements and Phasor Analyses.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Heterogeneity of CD4Frontiers in cell and developmental biology · 2020Article
- Fluorescence lifetime shifts of NAD(P)H during apoptosis measured by time-resolved flow cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Evaluating integrin activation with time-resolved flow cytometry.Journal of biomedical optics · 2018Article
- Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.Technology · 2018Article
- Shifts in the fluorescence lifetime of EGFP during bacterial phagocytosis measured by phase-sensitive flow cytometry.Scientific reports · 2017Article
- Phasor plotting with frequency-domain flow cytometry.Optics express · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Flow cytometry is a powerful means for in vitro cellular analyses where multi-fluorescence and multi-angle light scattering can indicate unique biochemical or morphological features of single cells. Yet, to date, flow cytometry systems have lacked the ability to capture complex fluorescence dynamics due to the transient nature of flowing cells. In this contribution we introduce a simple approach for measuring multiple fluorescence lifetimes from a single cytometric event. We leverage square wave modulation, Fourier analysis, and high frequency digitization and show the ability to resolve more than one fluorescence lifetime from fluorescently-labelled cells and microspheres. Illustration of a flow cytometer capable of capturing multiple fluorescence lifetime measurements; creating potential for multi-parametric, time-resolved signals to be captured for every color channel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.