ArticleCurrent protocols in chemical biology2015
Universal non-antibody detection of protein phosphorylation using pIMAGO.
Article in Current protocols in chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- GhTOPP4aD and GhRAF36 inversely regulate cotton (Gossypium hirsutum) response to ABA and salt stress through reversible phosphorylation of GhABI1.Plant biotechnology journal · 2025Article
- Type One Protein Phosphatase 4aD Negatively Regulates Cotton (International journal of molecular sciences · 2025Article
- Identification of Novel Kinases of Tau Using Fluorescence Complementation Mass Spectrometry (FCMS).Molecular & cellular proteomics : MCP · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
This article describes methods for a new, non-antibody phosphorylation detection reagent, termed pIMAGO (phospho-imaging). This novel reagent takes advantage not only of the unique properties of the soluble nanoparticles, but also of the multiple functionalities of the molecule, allowing for highly selective, sensitive, and quantitative assessment of protein phosphorylation without using radioactive isotopes or phospho-specific antibodies. The methods allow for multiplexed detection of phosphorylation and total protein amount simultaneously. The straightforward and routine detection and quantitation of general phosphorylation on any site of any protein can be performed in western blot and ELISA formats.
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Registered trials
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