Evidence map›Paper›PMID 25725517›Full record

ArticleMethods in enzymology2015

Chemiluminescent detection of enzymatically produced H2S.

T Spencer Bailey, Michael D Pluth

Abstract read
In one paragraph

Article in Methods in enzymology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

T Spencer BaileyDepartment of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, Oregon, USA.
Michael D PluthDepartment of Chemistry and Biochemistry, Institute of Molecular Biology, Materials Science Institute, University of Oregon, Eugene, Oregon, USA. Electronic address: pluth@uoregon.edu.

Funding

Developing fluorescent probes for the endogenous gaseous transmitters NO and H2SR00GM092970 · NIGMS · UNIVERSITY OF OREGON · PI PLUTH, MICHAEL · 2011 to 2013
$731k
NIGMS NIH HHS R00 GM092970
6 · The paper itself

Abstract

Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assist other investigators with use of these chemical tools. We highlight the use of these tools use by displaying their selectivity and high sensitivity toward H2S and provide examples of assays we have developed to detect enzymatically produced H2S.

Indexed as

AnimalsCell LineCystathionine gamma-LyaseEnzyme AssaysFluorescent DyesHydrogen SulfideLuminescent MeasurementsLuminolPhotochemical ProcessesRatsCystathionine gamma-LyaseFluorescent DyesHydrogen SulfideLuminolChemiluminescenceHydrogen sulfideLuminescenceLuminol

Identifiers

PMID25725517
PMCPMC4384472

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.