ArticleDevelopment (Cambridge, England)2015
Second-generation Notch1 activity-trap mouse line (N1IP::CreHI) provides a more comprehensive map of cells experiencing Notch1 activity.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Notch1 signaling is limited in healthy mature kidneys in vivo.BMC research notes · 2023Article
- Recent advances inFrontiers in cell and developmental biology · 2023Review
- Notch controls the cell cycle to define leader versus follower identities during collective cell migration.eLife · 2022Article
- SALSA, a genetically encoded biosensor for spatiotemporal quantification of Notch signal transduction in vivo.Developmental cell · 2022Article
- NOTCH1 signaling establishes the medullary thymic epithelial cell progenitor pool during mouse fetal development.Development (Cambridge, England) · 2020Article
- Notch1 haploinsufficiency causes ascending aortic aneurysms in mice.JCI insight · 2017Article
- Transient Notch Activation Induces Long-Term Gene Expression Changes Leading to Sick Sinus Syndrome in Mice.Circulation research · 2017Article
- Notch-Mediated Epigenetic Regulation of Voltage-Gated Potassium Currents.Circulation research · 2016Article
- Tissue Specific Expression of Cre in Rat Tyrosine Hydroxylase and Dopamine Active Transporter-Positive Neurons.PloS one · 2016Article
- Notch signal strength controls cell fate in the haemogenic endothelium.Nature communications · 2015Article
- The intracellular domains of Notch1 and Notch2 are functionally equivalent during development and carcinogenesis.Development (Cambridge, England) · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously described the creation and analysis of a Notch1 activity-trap mouse line, Notch1 intramembrane proteolysis-Cre6MT or N1IP::Cre(LO), that marked cells experiencing relatively high levels of Notch1 activation. Here, we report and characterize a second line with improved sensitivity (N1IP::Cre(HI)) to mark cells experiencing lower levels of Notch1 activation. This improvement was achieved by increasing transcript stability and by restoring the native carboxy terminus of Cre, resulting in a five- to tenfold increase in Cre activity. The magnitude of this effect probably impacts Cre activity in strains with carboxy-terminal Ert2 fusion. These two trap lines and the related line N1IP::Cre(ERT2) form a complementary mapping tool kit to identify changes in Notch1 activation patterns in vivo as the consequence of genetic or pharmaceutical intervention, and illustrate the variation in Notch1 signal strength from one tissue to the next and across developmental time.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.