Evidence map›Paper›PMID 25725012›Full record

ReviewFEMS microbiology reviews2015

Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface.

M Sloan Siegrist, Benjamin M Swarts, Douglas M Fox, Shion An Lim, Carolyn R Bertozzi

Abstract readReview
In one paragraph

Review in FEMS microbiology reviews, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed.

  1. Structure, assembly, and mechanism of the MmpL family of transporters.Microbiology and molecular biology reviews : MMBR · 2026
    Review
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  12. Imaging ofScience advances · 2023
    Article
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  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article

4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

M Sloan SiegristDepartment of Chemistry, University of California, Berkeley, CA 94720, USA.
Benjamin M SwartsDepartment of Chemistry, Central Michigan University, Mount Pleasant, MI 48859, USA.
Douglas M FoxDepartment of Chemistry, University of California, Berkeley, CA 94720, USA.
Shion An LimDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Carolyn R BertozziDepartment of Chemistry, University of California, Berkeley, CA 94720, USA Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA crb@berkeley.edu.

Funding

Univ of Calif Berkeley Chemistry-Biology Interface ProgramT32GM066698 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHANG, CHRISTOPHER J., FRANCIS, MATTHEW B · 2004 to 2023
$7.5M
Mycobacterial Sulfation PathwaysR01AI051622 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI BERTOZZI, CAROLYN · 2002 to 2015
$6.0M
NIAID NIH HHS 1R01AI051622NIAID NIH HHS R01 AI051622NIGMS NIH HHS T32 GM066698
6 · The paper itself

Abstract

The cell surface is the essential interface between a bacterium and its surroundings. Composed primarily of molecules that are not directly genetically encoded, this highly dynamic structure accommodates the basic cellular processes of growth and division as well as the transport of molecules between the cytoplasm and the extracellular milieu. In this review, we describe aspects of bacterial growth, division and secretion that have recently been uncovered by metabolic labeling of the cell envelope. Metabolite derivatives can be used to label a variety of macromolecules, from proteins to non-genetically-encoded glycans and lipids. The embedded metabolite enables precise tracking in time and space, and the versatility of newer chemoselective detection methods offers the ability to execute multiple experiments concurrently. In addition to reviewing the discoveries enabled by metabolic labeling of the bacterial cell envelope, we also discuss the potential of these techniques for translational applications. Finally, we offer some guidelines for implementing this emerging technology.

Indexed as

Staining and LabelingBacteriaBacteriological TechniquesCell Membranebioorthogonalclick chemistryglycolipidmetabolic labelingpeptidoglycanprotein secretion

Identifiers

PMID25725012
PMCPMC4462956

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.