ArticleMolecular & cellular proteomics : MCP2015
Proteomic analysis of the soybean symbiosome identifies new symbiotic proteins.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Symbiosome membrane-localized cationic amino acid transporters support symbiotic nitrogen fixation in Medicago truncatula.Plant communications · 2026Article
- Systemic proteome remodeling in soybean during early nodulation: distinct responses in roots and leaves.Frontiers in plant science · 2026Article
- Symbiosome functionality in Medicago truncatula nodules requires continuous clearing of pectins from the symbiosome space.Nature communications · 2025Article
- Review
- Single-cell transcriptome atlases of soybean root and mature nodule reveal new regulatory programs that control the nodulation process.Plant communications · 2024Article
- Metal nutrition and transport in the process of symbiotic nitrogen fixation.Plant communications · 2024Review
- NPF and NRT2 fromPlants (Basel, Switzerland) · 2024Review
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Genome-Wide Association Study of Soybean Germplasm Derived From Canadian × Chinese Crosses to Mine for Novel Alleles to Improve Seed Yield and Seed Quality Traits.Frontiers in plant science · 2022Article
- A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume-rhizobium symbioses.Plant physiology · 2021Review
- Malate Transport and Metabolism in Nitrogen-Fixing Legume Nodules.Molecules (Basel, Switzerland) · 2021Review
- PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.Plant physiology · 2021Article
- PHO1 proteins mediate phosphate transport in the legume-rhizobium symbiosis.Plant physiology · 2021Article
- Iron Transport across Symbiotic Membranes of Nitrogen-Fixing Legumes.International journal of molecular sciences · 2021Review
- TheFrontiers in plant science · 2021Article
- Nitrogen and Phosphorus Signaling and Transport During Legume-Rhizobium Symbiosis.Frontiers in plant science · 2021Review
- Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean.BMC plant biology · 2020Article
- The rhizobial autotransporter determines the symbiotic nitrogen fixation activity ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Understanding transport processes in lichen,F1000Research · 2020Review
- Convergent evolution of signal-structure interfaces for maintaining symbioses.Current opinion in microbiology · 2019Review
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing the symbiosome membrane (SM) and material from the lumen of the symbiosome (peribacteroid space or PBS) were isolated from soybean root nodules and analyzed using nongel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to reduce complexity of the samples and enrich for hydrophobic integral membrane proteins. One hundred and ninety-seven proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and PBS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and degradation, membrane trafficking, and solute transport were identified. These included a number of proteins previously localized to the SM, such as aquaglyceroporin nodulin 26, sulfate transporters, remorin, and Rab7 homologs. Among the proteome were a number of putative transporters for compounds such as sulfate, calcium, hydrogen ions, peptide/dicarboxylate, and nitrate, as well as transporters for which the substrate is not easy to predict. Analysis of the promoter activity for six genes encoding putative SM proteins showed nodule specific expression, with five showing expression only in infected cells. Localization of two proteins was confirmed using GFP-fusion experiments. The data have been deposited to the ProteomeXchange with identifier PXD001132. This proteome will provide a rich resource for the study of the legume-rhizobium symbiosis.
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