ArticleThe Plant cell2015
An upstream open reading frame is essential for feedback regulation of ascorbate biosynthesis in Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed, 226 citations in OpenAlex.
- Balancing tuber vitamin C improvement with trade-off side effect by fine-tuning regulation ofHorticulture research · 2026Article
- Ribosome stalling position, spacing, and A-site occupancy impact translation and cotranslational mRNA decay in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Monodehydroascorbate reductases in plants: integrating ascorbate recycling, redox signaling, and stress adaptation.Redox biology · 2026Review
- Identification of Potentially Coding Small ORFs in Plant Transcriptomes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Translational control in plants: from basic mechanisms to environmental and developmental responses.The Plant journal : for cell and molecular biology · 2026Review
- Simultaneous knockout of VITAMIN C DEFECTIVE 2 and 3 exacerbates ascorbate deficiency and light stress sensitivity.Journal of experimental botany · 2025Article
- Variations in the OsGGP uORF Fine-Tune Vitamin C Content and Confer Resistance to Osmotic Stress in Rice.Rice (New York, N.Y.) · 2025Article
- RNA elements and their biotechnological applications in plants.The New phytologist · 2025Review
- Article
- AcABI5a integrates abscisic acid signaling to developmentally modulate fruit ascorbic acid biosynthesis in kiwifruit.Horticulture research · 2025Article
- Combined enhancement of ascorbic acid, β-carotene and zeaxanthin in gene-edited lettuce.Plant biotechnology journal · 2025Article
- Tissue- and Condition-Specific Biosynthesis of Ascorbic Acid inInternational journal of molecular sciences · 2025Article
- Discovery of a conserved translationally repressive upstream open reading frame within the iron-deficiency response regulator IDEF2.BMC plant biology · 2024Article
- Article
- Characterization of the GGP gene family in potato (Solanum tuberosum L.) and Pepper (Capsicum annuum L.) and its expression analysis under hormonal and abiotic stresses.Scientific reports · 2024Article
- Ribosome decision graphs for the representation of eukaryotic RNA translation complexity.Genome research · 2024Article
- Revisiting the role of ascorbate oxidase in plant systems.Journal of experimental botany · 2024Review
- Ascorbic acid metabolism and functions.Journal of experimental botany · 2024Article
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
43 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascorbate (vitamin C) is an essential antioxidant and enzyme cofactor in both plants and animals. Ascorbate concentration is tightly regulated in plants, partly to respond to stress. Here, we demonstrate that ascorbate concentrations are determined via the posttranscriptional repression of GDP-l-galactose phosphorylase (GGP), a major control enzyme in the ascorbate biosynthesis pathway. This regulation requires a cis-acting upstream open reading frame (uORF) that represses the translation of the downstream GGP open reading frame under high ascorbate concentration. Disruption of this uORF stops the ascorbate feedback regulation of translation and results in increased ascorbate concentrations in leaves. The uORF is predicted to initiate at a noncanonical codon (ACG rather than AUG) and encode a 60- to 65-residue peptide. Analysis of ribosome protection data from Arabidopsis thaliana showed colocation of high levels of ribosomes with both the uORF and the main coding sequence of GGP. Together, our data indicate that the noncanonical uORF is translated and encodes a peptide that functions in the ascorbate inhibition of translation. This posttranslational regulation of ascorbate is likely an ancient mechanism of control as the uORF is conserved in GGP genes from mosses to angiosperms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.