ArticleJournal of virological methods2015
Production, purification, and capsid stability of rhinovirus C types.
Article in Journal of virological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Optimized Propagation and Purification Protocols for Large-Scale Production of Rhinovirus C.Viruses · 2026Article
- Method to Generate High-Titer Rhinovirus C Suitable for In Vitro and In Vivo Studies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Rhinovirus infection induces secretion of endothelin-1 from airway epithelial cells in both in vitro and in vivo models.Respiratory research · 2023Article
- Rhinoviruses A and C elicit long-lasting antibody responses with limited cross-neutralization.Journal of medical virology · 2023Article
- Rhinovirus C15 Attenuates Relaxation and cAMP Production in Human Airways and Smooth Muscle.American journal of respiratory cell and molecular biology · 2023Article
- Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium.PLoS pathogens · 2022Article
- Stability of SARS-CoV-2 and other airborne viruses under different stress conditions.Archives of virology · 2022Article
- Enhanced Neutralizing Antibody Responses to Rhinovirus C and Age-Dependent Patterns of Infection.American journal of respiratory and critical care medicine · 2021Article
- Rhinovirus C15 Induces Airway Hyperresponsiveness via Calcium Mobilization in Airway Smooth Muscle.American journal of respiratory cell and molecular biology · 2020Article
- Respiratory Enterovirus (like Parainfluenza Virus) Can Cause Chronic Lung Disease if Protection by Airway Epithelial STAT1 Is Lost.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- CDHR3 extracellular domains EC1-3 mediate rhinovirus C interaction with cells and as recombinant derivatives, are inhibitory to virus infection.PLoS pathogens · 2018Article
- Lethal Respiratory Disease Associated with Human Rhinovirus C in Wild Chimpanzees, Uganda, 2013.Emerging infectious diseases · 2018Article
- Rhinovirus C targets ciliated airway epithelial cells.Respiratory research · 2017Article
- Mutations in VP1 and 3A proteins improve binding and replication of rhinovirus C15 in HeLa-E8 cells.Virology · 2016Article
- Atomic structure of a rhinovirus C, a virus species linked to severe childhood asthma.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Virus/Allergen Interaction in Asthma Exacerbation.Annals of the American Thoracic Society · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The rhinovirus C (RV-C) were discovered in 2006 and these agents are an important cause of respiratory morbidity. Little is known about their biology. RV-C15 (C15) can be produced by transfection of recombinant viral RNA into cells and subsequent purification over a 30% sucrose cushion, even though yields and infectivity of other RV-C genotypes with this protocol are low. The goal of this study was to determine whether poor RV-C yields were due to capsid instability, and moreover, to develop a robust protocol suitable for the purification of many RV-C types. Capsid stability assays indicated that virions of RV-C41 (refractory to purification) have similar tolerance for osmotic and temperature stress as RV-A16 (purified readily), although C41 is more sensitive to low pH. Modification to the purification protocol by removing detergent increased the yield of RV-C. Addition of nonfat dry milk to the sucrose cushion increased the virus yield but sacrificed purity of the viral suspension. Analysis of virus distribution following centrifugation indicated that the majority of detectable viral RNA (vRNA) was found in pellets refractory to resuspension. Reduction of the centrifugal force with commiserate increase in spin-time improved the recovery of RV-C for both C41 and C2. Transfection of primary lung fibroblasts (WisL cells) followed by the modified purification protocol further improved yields of infectious C41 and C2. Described herein is a higher yield purification protocol suitable for RV-C types refractory to the standard purification procedure. The findings suggest that aggregation-adhesion problems rather than capsid instability influence RV-C yield during purification.
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Registered trials
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