Evidence map›Paper›PMID 25724426›Full record

ArticlePlant molecular biology2015

Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp. pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering.

Mei-Fang Song, Shu Zhang, Pei Hou, Hong-Zhong Shang, Hai-Ke Gu, Jing-Juan Li, Yang Xiao, Lin Guo, Liang Su, Jian-Wei Gao and 1 more

Abstract read
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In one paragraph

Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. International journal of molecular sciences · 2022
    Article
  4. Plant direct · 2022
    Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Mei-Fang SongBeijing Radiation Center, Beijing, 100875, People's Republic of China.
Shu Zhang
Pei Hou
Hong-Zhong Shang
Hai-Ke Gu
Jing-Juan Li
Yang Xiao
Lin Guo
Liang Su
Jian-Wei Gao
Jian-Ping Yang
Beijing Radiation Center · CNInstitute of Crop Sciences · CNInstitute of Vegetables and Flowers · CNChinese Academy of Agricultural Engineering · CNChinese Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.

Indexed as

Gene Expression Regulation, DevelopmentalArabidopsisArabidopsis ProteinsBase SequenceBrassica rapaEtiolationFlowersGene ExpressionGene Expression Regulation, PlantGibberellinsLightMolecular Sequence DataOrgan SpecificityPhylogenyPhytochrome BPlant Growth RegulatorsArabidopsis ProteinsGibberellinsPHYB protein, ArabidopsisPhytochrome BPlant Growth RegulatorsPlant Proteins

Identifiers

PMID25724426
OpenAlexW2055466338

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.