ArticleCurrent microbiology2015
Homocitrate synthase expression and lysine content in fruiting body of different developmental stages in Flammulina velutipes.
Article in Current microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 19 citations in OpenAlex.
- The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum.Microbial cell factories · 2023Article
- Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form.Journal of fungi (Basel, Switzerland) · 2023Article
- Molecular Evolution of Lysine Biosynthesis in Agaricomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Rapid and Accurate Screening of Lysine-Producing Edible Mushrooms via the Homocitrate Synthase Gene as a Universal Molecular Marker.ACS omega · 2021Article
- A putative transcription factor LFC1 negatively regulates development and yield of winter mushroom.Applied microbiology and biotechnology · 2020Article
- A WD40 Protein Encoding GeneFrontiers in microbiology · 2020Article
- A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom (Applied and environmental microbiology · 2019Article
- Comparative Transcriptomics ofInternational journal of molecular sciences · 2019Article
- A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes.International journal of molecular sciences · 2016Article
- Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusG3 (Bethesda, Md.) · 2016Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Homocitrate synthase (EC 2.3.3.14) regulates the first step of fungal lysine biosynthesis. The gene encoding homocitrate synthase was identified in whole genomic sequencing of Flammulina velutipes and contains seven introns. The homocitrate synthase gene of F. velutipes strain W23 (Fvhcs) is 1780 bp in length and encodes a 464 amino acid protein with a predicted molecular weight 50.7 kDa. Phylogenetic analysis of Fvhcs and other homocitrate synthase proteins from diverse fungi produced a topology congruent with the current best estimate of organismal phylogeny. Analysis of protein domains by InterProScan and a motif search found that Fvhcs gene encodes homocitrate synthase protein conserved across Agaricomycotina. In addition, we sequenced the transcriptome of different developmental stages and structures of the fruiting body to analyze the expression levels of the Fvhcs gene. The data showed a correlation between Fvhcs gene expression and lysine values in different developmental stages and structures of F. velutipes.
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