ArticleBiological research2014
Expression of non-neuronal cholinergic system in maxilla of rat in vivo.
Article in Biological research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Neural regulation of bone: from central neural circuits to peripheral innervation of the skeletal stem cell niche.Bone research · 2026Review
- Regulation of mild moxibustion on non-neuronal cholinergic system in ulcerative colitis rats.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2025Article
- Kidney organoid derived from renal tissue stem cells is a useful tool for histopathological assessment of nephrotoxicity in a cisplatin-induced acute renal tubular injury model.Journal of toxicologic pathology · 2022Article
- In vivo vesicular acetylcholine transporter density in human peripheral organs: an [EJNMMI research · 2022Article
- Non-neuronal Role of Acetylcholinesterase in Bone Development and Degeneration.Frontiers in cell and developmental biology · 2020Review
- Deletion of nicotinic acetylcholine receptor alpha9 in mice resulted in altered bone structure.Bone · 2019Article
- Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis.Journal of neuroimmunology · 2018Article
- Involvement of the Nonneuronal Cholinergic System in Bone Remodeling in Rat Midpalatal Suture after Rapid Maxillary Expansion.BioMed research international · 2016Article
- Acetylcholinesterase Regulates Skeletal In Ovo Development of Chicken Limbs by ACh-Dependent and -Independent Mechanisms.PloS one · 2016Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study.
resultsComponents of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, β1, β2, β4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits α2, β1, β4 and mAChR subunits M4 had abundant expression (2(-ΔCt) > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells.
conclusionsACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.
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