ArticleThe Biochemical journal2015
Assembly and structure of Lys33-linked polyubiquitin reveals distinct conformations.
Article in The Biochemical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- E3 ligase AREL1 controls perinuclear localization of lysosomes and supports Purkinje cell survival.The EMBO journal · 2026Article
- The role of HECT-type E3 ubiquitin ligases in DNA damage response and repair.Cell death discovery · 2025Review
- IL-1β turnover by the UBE2L3 ubiquitin conjugating enzyme and HECT E3 ligases limits inflammation.Nature communications · 2023Article
- TheActa biochimica et biophysica Sinica · 2023Article
- TRABID overexpression enables synthetic lethality to PARP inhibitor via prolonging 53BP1 retention at double-strand breaks.Nature communications · 2023Article
- The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis.Scientific reports · 2022Article
- The chemical biology of ubiquitin.Biochimica et biophysica acta. General subjects · 2022Review
- Mechanism and Disease Association With a Ubiquitin Conjugating E2 Enzyme: UBE2L3.Frontiers in immunology · 2022Review
- Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2.Molecular cell · 2021Article
- Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles.Nature communications · 2021Article
- Structure, Dynamics and Function of the 26S Proteasome.Sub-cellular biochemistry · 2021Review
- Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies.PloS one · 2021Article
- Hepatitis B Core Protein Is Post-Translationally Modified through K29-Linked Ubiquitination.Cells · 2020Article
- The Ubiquitin Conjugating Enzyme: An Important Ubiquitin Transfer Platform in Ubiquitin-Proteasome System.International journal of molecular sciences · 2020Review
- Unanchored Ubiquitin Chains, Revisited.Frontiers in cell and developmental biology · 2020Review
- E3 Ubiquitin ligase NEDD4 family‑regulatory network in cardiovascular disease.International journal of biological sciences · 2020Review
- Structural insights into a HECT-type E3 ligase AREL1 and its ubiquitination activitiesThe Journal of biological chemistry · 2019Article
- KIAA0317 regulates pulmonary inflammation through SOCS2 degradation.JCI insight · 2019Article
- Synthesis of Branched Triubiquitin Active-Site Directed Probes.Organic letters · 2019Article
- Analysis of ubiquitin recognition by the HECT ligase E6AP provides insight into its linkage specificity.The Journal of biological chemistry · 2019Article
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8 authors.
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Abstract
Ubiquitylation regulates a multitude of biological processes and this versatility stems from the ability of ubiquitin (Ub) to form topologically different polymers of eight different linkage types. Whereas some linkages have been studied in detail, other linkage types including Lys33-linked polyUb are poorly understood. In the present study, we identify an enzymatic system for the large-scale assembly of Lys33 chains by combining the HECT (homologous to the E6-AP C-terminus) E3 ligase AREL1 (apoptosis-resistant E3 Ub protein ligase 1) with linkage selective deubiquitinases (DUBs). Moreover, this first characterization of the chain selectivity of AREL1 indicates its preference for assembling Lys33- and Lys11-linked Ub chains. Intriguingly, the crystal structure of Lys33-linked diUb reveals that it adopts a compact conformation very similar to that observed for Lys11-linked diUb. In contrast, crystallographic analysis of Lys33-linked triUb reveals a more extended conformation. These two distinct conformational states of Lys33-linked polyUb may be selectively recognized by Ub-binding domains (UBD) and enzymes of the Ub system. Importantly, our work provides a method to assemble Lys33-linked polyUb that will allow further characterization of this atypical chain type.
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