ArticlePloS one2015
Identification and deletion of Tft1, a predicted glycosyltransferase necessary for cell wall β-1,3;1,4-glucan synthesis in Aspergillus fumigatus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Plant cell walls as a key driver of plant-pathogen coevolution.FEMS microbiology reviews · 2026Review
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- Comparative analysis of polysaccharide and cell wall structure in Aspergillus nidulans and Aspergillus fumigatus by solid-state NMR.Carbohydrate polymers · 2025Article
- Comparative Analysis of Polysaccharide and Cell Wall Structure inbioRxiv : the preprint server for biology · 2024Article
- Adaptative survival of Aspergillus fumigatus to echinocandins arises from cell wall remodeling beyond β-1,3-glucan synthesis inhibition.Nature communications · 2024Article
- Glucanases and Chitinases inMolecules (Basel, Switzerland) · 2024Article
- The Gram-positive bacterium Romboutsia ilealis harbors a polysaccharide synthase that can produce (1,3;1,4)-β-D-glucans.Nature communications · 2023Article
- Chromosome-level genomes of multicellular algal sisters to land plants illuminate signaling network evolution.bioRxiv : the preprint server for biology · 2023Article
- Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- Cell wall-derived mixed-linked β-1,3/1,4-glucans trigger immune responses and disease resistance in plants.The Plant journal : for cell and molecular biology · 2021Article
- Review
- Ultrasound Imaging of Cervical Anatomic Variants.Current medical imaging · 2021Article
- Galactomannan Produced byJournal of fungi (Basel, Switzerland) · 2020Review
- Article
- Review
- Article
- The Fungal Cell Wall:Frontiers in microbiology · 2019Review
- Functional Characterization of a Glycosyltransferase from the MossThe Plant cell · 2018Article
- Diversity of Cell Wall Related Proteins in Human Pathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2017Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus fumigatus is an environmental mold that causes severe, often fatal invasive infections in immunocompromised patients. The search for new antifungal drug targets is critical, and the synthesis of the cell wall represents a potential area to find such a target. Embedded within the main β-1,3-glucan core of the A. fumigatus cell wall is a mixed linkage, β-D-(1,3;1,4)-glucan. The role of this molecule or how it is synthesized is unknown, though it comprises 10% of the glucans within the wall. While this is not a well-studied molecule in fungi, it has been studied in plants. Using the sequences of two plant mixed linkage glucan synthases, a single ortholog was identified in A. fumigatus (Tft1). A strain lacking this enzyme (tft1Δ) was generated along with revertant strains containing the native gene under the control of either the native or a strongly expressing promoter. Immunofluorescence staining with an antibody against β-(1,3;1,4)-glucan and biochemical quantification of this polysaccharide in the tft1Δ strain demonstrated complete loss of this molecule. Reintroduction of the gene into the knockout strain yielded reappearance in amounts that correlated with expected expression of the gene. The loss of Tft1 and mixed linkage glucan yielded no in vitro growth phenotype. However, there was a modest increase in virulence for the tft1Δ strain in a wax worm model. While the precise roles for β-(1,3;1,4)-glucan within A. fumigatus cell wall are still uncertain, it is clear that Tft1 plays a pivotal role in the biosynthesis of this cell wall polysaccharide.
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