ArticleBrain and behavior2015
Membrane potential depolarization causes alterations in neuron arrangement and connectivity in cocultures.
Article in Brain and behavior, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
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- Analysis of the Underlying Mechanism of the Jiu Wei Zhen Xin Formula for Treating Generalized Anxiety Disorder Based on Network Pharmacology of Traditional Chinese Medicine.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Review
- Bioelectrical controls of morphogenesis: from ancient mechanisms of cell coordination to biomedical opportunities.Current opinion in genetics & development · 2019Review
- Permethrin and ivermectin modulate lipid metabolism in steatosis-induced HepG2 hepatocyte.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2019Article
- EDEn-Electroceutical Design Environment: Ion Channel Tissue Expression Database with Small Molecule Modulators.iScience · 2019Article
- Long-Term, Stochastic Editing of Regenerative Anatomy via Targeting Endogenous Bioelectric Gradients.Biophysical journal · 2017Article
- Endogenous Two-Photon Excited Fluorescence Imaging Characterizes Neuron and Astrocyte Metabolic Responses to Manganese Toxicity.Scientific reports · 2017Article
- A biofidelic 3D culture model to study the development of brain cellular systems.Scientific reports · 2016Article
- Re-membering the body: applications of computational neuroscience to the top-down control of regeneration of limbs and other complex organs.Integrative biology : quantitative biosciences from nano to macro · 2015Review
- Automatic neuron segmentation and neural network analysis method for phase contrast microscopy images.Biomedical optics express · 2015Article
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Authors and funding
8 authors.
Funding
Abstract
backgroundThe disruption of neuron arrangement is associated with several pathologies. In contrast to action potentials, the role of resting potential (Vmem) in regulating connectivity remains unknown.
methodsNeuron assemblies were quantified when their Vmem was depolarized using ivermectin (Ivm), a drug that opens chloride channels, for 24 h in cocultures with astrocytes. Cell aggregation was analyzed using automated cluster analysis methods. Neural connectivity was quantified based on the identification of isolated somas in phase-contrast images using image processing. Vmem was measured using voltage-sensitive dyes and whole-cell patch clamping. Immunocytochemistry and Western blotting were used to detect changes in the distribution and production of the proteins.
resultsData show that Vmem regulates cortical tissue shape and connectivity. Automated cluster analysis methods revealed that the degree of neural aggregation was significantly increased (0.26 clustering factor vs. 0.21 in controls, P ≤ 0.01). The number of beta-tubulin III positive neural projections was also significantly increased in the neural aggregates in cocultures with Ivm. Hyperpolarized neuron cells formed fewer connections (33% at 24 h, P ≤ 0.05) compared to control cells in 1-day cultures. Glia cell densities increased (33.3%, P ≤ 0.05) under depolarizing conditions.
conclusionVmem can be a useful tool to probe neuronal cells, disease tissues models, and cortical tissue arrangements.
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