ArticlePathogens and disease2015
DNA methylation differentially regulates cytokine secretion in gingival epithelia in response to bacterial challenges.
Article in Pathogens and disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Association of Salivary Micro Ribonucleic Acid Levels With the Severity of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Children.Pediatric discovery · 2026Article
- Article
- Important role of DNA methylation hints at significant potential in tuberculosis.Archives of microbiology · 2024Review
- Saliva microRNA Profile in Children with and without Severe SARS-CoV-2 Infection.International journal of molecular sciences · 2023Article
- Porphyromonas gingivalis outside the oral cavity.Odontology · 2022Review
- Inhibitors of DNA Methylation.Advances in experimental medicine and biology · 2022Review
- Outer membrane vesicles from bacteria: Role and potential value in the pathogenesis of chronic respiratory diseases.Frontiers in cellular and infection microbiology · 2022Review
- Temporal genome-wide DNA methylation signature of post-smolt Pacific salmon challenged withEpigenetics · 2021Article
- Epigenetic regulation of inflammation in periodontitis: cellular mechanisms and therapeutic potential.Clinical epigenetics · 2020Review
- [Advances in oral bacteria influencing host epigenetic regulation].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020Review
- Host Epigenetics in Intracellular Pathogen Infections.International journal of molecular sciences · 2020Review
- Role of epigenetics in modulation of immune response at the junction of host-pathogen interaction and danger molecule signaling.Pathogens and disease · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Epigenetic modifications are changes in gene expression without altering DNA sequence. We previously reported that bacteria-specific innate immune responses are regulated by epigenetic modifications. Our hypothesis is that DNA methylation affects gingival cytokine secretion in response to bacterial stimulation. Gingival epithelial cells (GECs) were treated with DNMT-1 inhibitors prior to Porphyromonas gingivalis (Pg) or Fusobacterium nucleatum (Fn) exposure. Protein secretion was assessed using ELISA. Gene expression was quantified using qRT-PCR. The ability of bacteria to invade inhibitor pretreated GECs was assessed utilizing flow cytometry. Changes were compared to unstimulated GECs. GEC upregulation of IL-6 and CXCL1 by Pg or Fn stimulation was significantly diminished by inhibitor pretreatment. Pg stimulated IL-1α secretion and inhibitor pretreatment significantly enhanced this upregulation, while Fn alone or with inhibitor pretreatment had no effect on IL-1α expression. GEC upregulation of human beta-definsin-2 in response to Pg and Fn exposure was enhanced following the inhibitor pretreatment. GEC susceptibility to bacterial invasion was unaltered. These results suggest that DNA methylation differentially affects gingival cytokine secretion in response to Pg or Fn. Our data provide basis for better understanding of how epigenetic modifications, brought on by exposure to oral bacteria, will subsequently affect host susceptibility to oral diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.