ArticleNucleic acids research2015
Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Transcription-replication collisions trigger high-fidelity replication reset.Nucleic acids research · 2025Article
- Genome-wide mapping of spontaneous DNA replication error-hotspots using mismatch repair proteins in rapidly proliferating Escherichia coli.Nucleic acids research · 2025Article
- Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.Theory in biosciences = Theorie in den Biowissenschaften · 2024Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Robust linear DNA degradation supports replication-initiation-defective mutants in Escherichia coli.G3 (Bethesda, Md.) · 2022Article
- Article
- Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli.Scientific reports · 2021Article
- Generation of Bacterial Diversity by Segregation of DNA Strands.Frontiers in microbiology · 2021Article
- Nucleoid-mediated positioning and transport in bacteria.Current genetics · 2020Review
- Article
- The bacterial replisome has factory-like localization.Current genetics · 2018Review
- Fundamental principles in bacterial physiology-history, recent progress, and the future with focus on cell size control: a review.Reports on progress in physics. Physical Society (Great Britain) · 2018Review
- SeqA structures behind Escherichia coli replication forks affect replication elongation and restart mechanisms.Nucleic acids research · 2017Article
- Probing bacterial cell biology using image cytometry.Molecular microbiology · 2017Article
- Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter.The EMBO journal · 2017Article
- Review
- Lack of the H-NS Protein Results in Extended and Aberrantly Positioned DNA during Chromosome Replication and Segregation in Escherichia coli.Journal of bacteriology · 2016Article
- Stochasticity and homeostasis in the E. coli replication and division cycle.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Escherichia coli SeqA protein binds to newly replicated, hemimethylated DNA behind replication forks and forms structures consisting of several hundred SeqA molecules bound to about 100 kb of DNA. It has been suggested that SeqA structures either direct the new sister DNA molecules away from each other or constitute a spacer that keeps the sisters together. We have developed an image analysis script that automatically measures the distance between neighboring foci in cells. Using this tool as well as direct stochastic optical reconstruction microscopy (dSTORM) we find that in cells with fluorescently tagged SeqA and replisome the sister SeqA structures were situated close together (less than about 30 nm apart) and relatively far from the replisome (on average 200-300 nm). The results support the idea that newly replicated sister molecules are kept together behind the fork and suggest the existence of a stretch of DNA between the replisome and SeqA which enjoys added stabilization. This could be important in facilitating DNA transactions such as recombination, mismatch repair and topoisomerase activity. In slowly growing cells without ongoing replication forks the SeqA protein was found to reside at the fully methylated origins prior to initiation of replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.