ArticleNucleic acids research2015
SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Single-cell RNA sequencing offers novel perspectives in viral infection research.Frontiers in cellular and infection microbiology · 2026Pooled it
- Integrative single-cell and spatial transcriptomic approaches to decipher the tumor microenvironment and therapeutic resistance in pancreatic cancer.Cancer biology & therapy · 2026Review
- Filamin-ETV4/5 acts as mechanosensor-mechanotransducer axis that drives cell competition-mediated elimination of transformed cells.Nature communications · 2026Article
- Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.bioRxiv : the preprint server for biology · 2026Article
- The mitotic STAG3-cohesin complex shapes male germline nucleome.Nature structural & molecular biology · 2025Article
- Mouse trophectoderm stem cells generated with morula signalling inducers capture an early trophectoderm state.Nature cell biology · 2025Article
- Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in early mouse embryos.eLife · 2025Article
- Hallmarks of uterine receptivity predate placental mammals.bioRxiv : the preprint server for biology · 2024Article
- Article
- Integration of single-cell transcriptome and chromatin accessibility and its application on tumor investigation.Life medicine · 2024Review
- Effects of blastocyst elongation and implantation chamber formation on the alignment of the embryonic axis and uterine axis in mice.Frontiers in cell and developmental biology · 2024Article
- CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development.PLoS genetics · 2023Article
- High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesis.Life science alliance · 2023Article
- Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys.The EMBO journal · 2023Article
- Loss of Dead end1 induces testicular teratomas from primordial germ cells that failed to undergo sexual differentiation in embryonic testes.Scientific reports · 2023Article
- The Evolution of Single-Cell RNA Sequencing Technology and Application: Progress and Perspectives.International journal of molecular sciences · 2023Review
- Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells.eLife · 2023Article
- Reconstitution of human adrenocortical specification and steroidogenesis using induced pluripotent stem cells.Developmental cell · 2022Article
- Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys.The EMBO journal · 2022Article
- The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling.Stem cell reports · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell mRNA sequencing (RNA-seq) methods have undergone rapid development in recent years, and transcriptome analysis of relevant cell populations at single-cell resolution has become a key research area of biomedical sciences. We here present single-cell mRNA 3-prime end sequencing (SC3-seq), a practical methodology based on PCR amplification followed by 3-prime-end enrichment for highly quantitative, parallel and cost-effective measurement of gene expression in single cells. The SC3-seq allows excellent quantitative measurement of mRNAs ranging from the 10,000-cell to 1-cell level, and accordingly, allows an accurate estimate of the transcript levels by a regression of the read counts of spike-in RNAs with defined copy numbers. The SC3-seq has clear advantages over other typical single-cell RNA-seq methodologies for the quantitative measurement of transcript levels and at a sequence depth required for the saturation of transcript detection. The SC3-seq distinguishes four distinct cell types in the peri-implantation mouse blastocysts. Furthermore, the SC3-seq reveals the heterogeneity in human-induced pluripotent stem cells (hiPSCs) cultured under on-feeder as well as feeder-free conditions, demonstrating a more homogeneous property of the feeder-free hiPSCs. We propose that SC3-seq might be used as a powerful strategy for single-cell transcriptome analysis in a broad range of investigations in biomedical sciences.
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