Evidence map›Paper›PMID 25721972›Full record

ArticleCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology2015

Genistein stimulates jejunum chloride secretion via an Akt-mediated pathway in intact female mice.

Lana Leung, Ashesh Bhakta, Katherine Cotangco, Layla Al-Nakkash

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Soy Isoflavones and Gastrointestinal Health.Current nutrition reports · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Lana LeungDepartment of Physiology, Midwestern University, Glendale, AZ, USA.
Ashesh Bhakta
Katherine Cotangco
Layla Al-Nakkash
Midwestern University · US

Funding

Daily genistein injections stimulate intestinal secretion: How?R15DK071625 · NIDDK · MIDWESTERN UNIVERSITY · PI AL-NAKKASH, LAYLA · 2007 to 2007
$193k
NIDDK NIH HHS R15 DK071625NIDDK NIH HHS R15-DK071625-01A2
6 · The paper itself

Abstract

BACKGROUND/

aimsWe have previously shown that daily subcutaneous injections with the naturally occurring phytoestrogen genistein (600 mg genistein/kg body weight/day, 600G) results in a significantly increased basal intestinal chloride, Cl(-), secretion (Isc, a measure of transepithelial secretion) in intact C57BL/6J female mice after 1-week of treatment, compared to controls (DMSO vehicle injected). Removal of endogenous estrogen via ovariectomy (OVX) had no effect on the 600G-mediated increase in basal Isc.

methodsGiven the estrogen-like characteristics of genistein, we compared the effects of daily estradiol (E2) injections (10 mg E2/kg body weight/day, 10E2) on basal Isc in intact and OVX mice. In intact mice, 10E2 was without effect on basal Isc, however, in OVX mice, 10E2 significantly increased basal Isc (mimicked 600G). The goal of the current study was to characterize the intracellular signaling pathways responsible for mediating 600G- or 10E2-stimulated increases in basal Isc in intact female or OVX mice.

resultsWe measured total protein expression in isolated segments of jejunum using western blot from the following six groups of mice; intact or OVX with; 600G, 10E2 or control. The proteins of interest were: Akt, p-Akt, p-PDK1, p-PTEN, p-c-Raf, p-GSK-3β, rap-1 and ERK1/2. All blots were normalized to GAPDH levels (n = 6-18/group).

conclusionThese data suggest that the presence of the endogenous sex steroid, estrogen, modifies the intracellular signaling pathway required to mediate Cl(-) secretion when the intestine is exposed to exogenous 600G or E2. These studies may have relevance for designing pharmacological tools for women with intestinal chloride secretory dysfunctions.

Indexed as

AnimalsAnticarcinogenic AgentsBlotting, WesternChloridesEstradiolFemaleGenisteinGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaJejunumMiceMice, Inbred C57BLMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3OvariectomyPhosphorylationAnticarcinogenic AgentsChloridesEstradiolGenisteinGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaGsk3b protein, mouseMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Pdk1 protein, mouseProtein Serine-Threonine KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-rafPyruvate Dehydrogenase Acetyl-Transferring Kinaserap1 GTP-Binding Proteins

Identifiers

PMID25721972
PMCPMC4386721
OpenAlexW1975795090

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.