ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2015
Actin cytoskeleton contributes to the elastic modulus of embryonic tendon during early development.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Directed Cell Self-Assembly to Form Tendon and Muscle Models for Studying Early Stages of Musculoskeletal Tissue Formation.Journal of biomechanical engineering · 2026Article
- Imaging the mechanical properties of a developing embryonic tendon using Brillouin microscopy.Acta biomaterialia · 2025Article
- Focal adhesion kinase regulates tendon cell mechanoresponse and physiological tendon development.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Cell spheroid viscoelasticity is deformation-dependent.Scientific reports · 2024Article
- Recombinant lysyl oxidase effects on embryonic tendon cell phenotype and behavior.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Establishing in vivo and ex vivo chick embryo models to investigate fetal tendon healing.Scientific reports · 2023Article
- High-throughput mechanophenotyping of multicellular spheroids using a microfluidic micropipette aspiration chip.Lab on a chip · 2023Article
- Phenotype stability, expansion potential, and senescence of embryonic tendon cells in vitro.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Regulators of collagen crosslinking in developing and adult tendons.European cells & materials · 2022Review
- Method development and characterization of chick embryo tendon mechanical properties.Journal of biomechanics · 2022Article
- Craniofacial tendon development-Characterization of extracellular matrix morphology and spatiotemporal protein distribution.Frontiers in cell and developmental biology · 2022Article
- Article
- Stretch-Induced Tenomodulin Expression Promotes Tenocyte Migration via F-Actin and Chromatin Remodeling.International journal of molecular sciences · 2021Article
- Basic Structure, Physiology, and Biochemistry of Connective Tissues and Extracellular Matrix Collagens.Advances in experimental medicine and biology · 2021Article
- Interplay of Forces and the Immune Response for Functional Tendon Regeneration.Frontiers in cell and developmental biology · 2021Review
- 3D Bioprinting of Human Adipose-Derived Stem Cells and Their Tenogenic Differentiation in Clinical-Grade Medium.International journal of molecular sciences · 2020Article
- Tendon Biomechanics and Crimp Properties Following Fatigue Loading Are Influenced by Tendon Type and Age in Mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Cell-cell junctions in developing and adult tendons.Tissue barriers · 2020Article
- Design of a Bioreactor to Assess the Effect of Passive Joint Loading in a Live Chick EmbryoTissue engineering. Part C, Methods · 2019Article
- TGFβ2-induced tenogenesis impacts cadherin and connexin cell-cell junction proteins in mesenchymal stem cells.Biochemical and biophysical research communications · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Tendon injuries are common and heal poorly. Strategies to regenerate or replace injured tendons are challenged by an incomplete understanding of normal tendon development. Our previous study showed that embryonic tendon elastic modulus increases as a function of developmental stage. Inhibition of enzymatic collagen crosslink formation abrogated increases in tendon elastic modulus at late developmental stages, but did not affect increases in elastic modulus of early stage embryonic tendons. Here, we aimed to identify potential contributors to the mechanical properties of these early stage embryonic tendons. We characterized tendon progenitor cells in early stage embryonic tendons, and the influence of actin cytoskeleton disruption on tissue elastic modulus. Cells were closely packed in embryonic tendons, and did not change in density during early development. We observed an organized network of actin filaments that seemed contiguous between adjacent cells. The actin filaments exhibited a crimp pattern with a period and amplitude that matched the crimp of collagen fibers at each developmental stage. Chemical disruption of the actin cytoskeleton decreased tendon tissue elastic modulus, measured by atomic force microscopy. Our results demonstrate that early developmental stage embryonic tendons possess a well organized actin cytoskeleton network that contributes significantly to tendon tissue mechanical properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.