ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2015
Optical reconstruction of murine colorectal mucosa at cellular resolution.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Wound-healing plasticity enables clonal expansion of founder progenitor cells in colitis.Developmental cell · 2023Article
- Article
- Frequent Intraluminal Growth of Large Muscular Veins in Surgically Resected Colorectal Cancer Tissues: A 3-Dimensional Pathologic Reconstruction Study.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2023Article
- Transitional Anal Cells Mediate Colonic Re-epithelialization in Colitis.Gastroenterology · 2022Article
- Persistence of Lgr5+ colonic epithelial stem cells in mouse models of inflammatory bowel disease.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Gastrointestinal biofilms in health and disease.Nature reviews. Gastroenterology & hepatology · 2021Review
- Optical clearing reveals TNBS-induced morphological changes of VGLUT2-positive nerve fibers in mouse colorectum.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Cellular maps of gastrointestinal organs: getting the most from tissue clearing.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Optical clearing of small intestine for three-dimensional visualization of cellular proliferation within crypts.Journal of anatomy · 2018Article
- The Gut Microbiome: Connecting Spatial Organization to Function.Cell host & microbe · 2017Review
- High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The mucosal layer of the colon is a unique and dynamic site where host cells interface with one another and the microbiome, with major implications for physiology and disease. However, the cellular mechanisms mediating colonic regeneration, inflammation, dysplasia, and dysbiosis remain undercharacterized, partly because the use of thin tissue sections in many studies removes important volumetric context. To address these challenges in visualization, we have developed the deep mucosal imaging (DMI) method to reconstruct continuous extended volumes of mouse colorectal mucosa at cellular resolution. Use of ScaleA2 and SeeDB clearing agents enabled full visualization of the colonic crypt, the fundamental unit of adult colon. Confocal imaging of large colorectal expanses revealed epithelial structures involved in repair, inflammation, tumorigenesis, and stem cell function, in fluorescent protein-labeled, immunostained, paraffin-embedded, or human biopsy samples. We provide freely available software to reconstruct and explore on computers with standard memory allocations the large DMI datasets containing in toto representations of distal colonic mucosal volume. Extended-volume imaging of colonic mucosa through the novel, extensible, and readily adopted DMI approach will expedite mechanistic investigations of intestinal physiology and pathophysiology at intracrypt to multicrypt length scales.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.