Evidence map›Paper›PMID 25721054›Full record

ArticleJournal of insect physiology2015

Detection of insemination status in live Aedes aegypti females.

María C Carrasquilla, L Philip Lounibos

Abstract read
In one paragraph

Article in Journal of insect physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Single-Pair and Small-Group Crosses ofCold Spring Harbor protocols · 2023
    Article
  4. Article
  5. Determining the Insemination Status ofCold Spring Harbor protocols · 2022
    Article
  6. Insemination inCold Spring Harbor protocols · 2022
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

María C CarrasquillaFlorida Medical Entomology Laboratory, University of Florida, 200 9th Street S.E., Vero Beach, FL 32962, USA. Electronic address: mccarrasquilla@ufl.edu.
L Philip LounibosFlorida Medical Entomology Laboratory, University of Florida, 200 9th Street S.E., Vero Beach, FL 32962, USA.

Funding

Reproductive interference between invasive Aedes vectorsR21AI095780 · NIAID · UNIVERSITY OF FLORIDA · PI LOUNIBOS, LEON PHILIP · 2011 to 2012
$403k
NIAID NIH HHS R21 AI095780
6 · The paper itself

Abstract

Using the technique described in this report, the presence or absence of sperm in spermathecae of female Aedes aegypti is detectable without dissection. Spermathecae of a lightly anesthetized female can be visualized by phase contrast microscopy through the distended abdomen, after the intersegmental membranes are stretched by ventral placement of a glass cover slip. Most females recovered after the procedure were capable of subsequent reproductive activities. Albeit tedious, this technique preserves the female alive for subsequent experiments or observations. Its extension to other mosquito species, or other Diptera and insects, will depend on spermathecal and sperm visibility through the distended abdomen.

Indexed as

AedesAnimalsFemaleGenitalia, FemaleInseminationMaleMicroscopy, Phase-ContrastSpermatozoaAedes aegyptiInsemination statusLiveNew technique

Identifiers

PMID25721054
PMCPMC4385415

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.