Evidence map›Paper›PMID 25721051›Full record

ArticleJournal of medical microbiology2015

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains.

Camila Hamond, Melissa Pinna, Marco Alberto Medeiros, Pascale Bourhy, Walter Lilenbaum, Mathieu Picardeau

Abstract readEvaluation Study
In one paragraph

Article in Journal of medical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Microorganisms · 2026
    Review
  2. Transboundary and emerging diseases · 2026
    Article
  3. Review
  4. Comparative genomics ofFrontiers in microbiology · 2024
    Article
  5. Article
  6. Bovine Leptospirosis Due to Persistent Renal Carriage ofFrontiers in veterinary science · 2022
    Article
  7. Article
  8. Frontiers in veterinary science · 2022
    Article
  9. Of Mice, Cattle, and Men: A Review of the Eco-Epidemiology ofTropical medicine and infectious disease · 2021
    Review
  10. Article
  11. Article
  12. Molecular diagnostics for human leptospirosis.Current opinion in infectious diseases · 2016
    Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Camila HamondLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Melissa PinnaDepartment of Preventive Veterinary Medicine and Animal Production, Universidade Federal da Bahia, Salvador, BA, Brazil.
Marco Alberto MedeirosBio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, RJ, Brazil.
Pascale BourhyInstitut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France.
Walter LilenbaumLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Mathieu PicardeauInstitut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France.

Funding

NIAID NIH HHS HHSN272200900007C
6 · The paper itself

Abstract

Considering the prevalence of Leptospira santarosai infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from L. santarosai genome sequences resulted in a wide range of sizes for the amplified products amongst the 21 L. santarosai strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. secY sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between L. santarosai isolates, and this new typing tool could be used to investigate leptospirosis in regions where L. santarosai predominates.

Indexed as

Minisatellite RepeatsAmericasAnimalsGenetic VariationHumansLeptospiraLeptospirosisMolecular TypingSequence Analysis, DNA

Identifiers

PMID25721051
PMCPMC4857445

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.