Evidence map›Paper›PMID 25720657›Full record

ArticleExperimental eye research2015

Thermally labile components of aqueous humor potently induce osteogenic potential in adipose-derived mesenchymal stem cells.

Joshua T Morgan, Heung Sun Kwon, Joshua A Wood, Dori L Borjesson, Stanislav I Tomarev, Christopher J Murphy, Paul Russell

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Joshua T MorganDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, USA.
Heung Sun KwonSection of Retinal Ganglion Cell Biology, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD, USA.
Joshua A WoodDepartment of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA.
Dori L BorjessonDepartment of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA.
Stanislav I TomarevSection of Retinal Ganglion Cell Biology, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD, USA.
Christopher J MurphyDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, USA; Department of Ophthalmology & Vision Science, School of Medicine, University of California, Davis, USA.
Paul RussellDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, USA. Electronic address: prussell@ucdavis.edu.
University of California, Davis · USNational Eye Institute · US

Funding

Molecular Mechanisms Of GlaucomaZIAEY000318 · NEI · NATIONAL EYE INSTITUTE · PI TOMAREV, STANISLAV I · 2009 to 2025
$18.9M
Biophysical Cues and Corneal Wound HealingR01EY019970 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MURPHY, CHRISTOPHER JOHN · 2011 to 2020
$3.5M
Role of Biophysical Cues in the Pathogenesis of GlaucomaR01EY019475 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI RUSSELL, PAUL · 2010 to 2013
$1.5M
Intramural NIH HHSNEI NIH HHS R01 EY019475NEI NIH HHS R01EY019475NEI NIH HHS R01 EY019970NEI NIH HHS R01EY019970
6 · The paper itself

Abstract

Adipose-derived mesenchymal stem cells (ASCs) hold promise for use in cell-based therapies. Their intrinsic anti-inflammatory properties are potentially useful for treatments of inflammatory conditions such as uveitis, while their ability to differentiate along multiple cell lineages suggests use in regenerating damaged or degenerated tissue. However, how ASCs will respond to the intraocular environment is poorly studied. We have recently reported that aqueous humor (AH), the fluid that nourishes the anterior segment of the eye, potently increases alkaline phosphatase (ALP) activity of ASCs, indicating osteogenic differentiation. Here, we expand on our previous findings to better define the nature of this response. To this end, we cultured ASCs in the presence of 0, 5, 10, and 20% AH and assayed them for ALP activity. We found ALP activity correlates with increasing AH concentrations from 5 to 20%, and that longer treatments result in increased ALP activity. By using serum free media and pretreating AH with dextran-coated charcoal, we found that serum and charcoal-adsorbable AH components augment but are not required for this response. Further, by heat-treating the AH, we established that thermally labile components are required for the osteogenic response. Finally, we showed myocilin, a protein present in AH, could induce ALP activity in ASCs. However, this was to a lesser extent than untreated 5% AH, and myocilin could only partially rescue the effect after heat treatment, documenting there were additional thermally labile constituents of AH involved in the osteogenic response. Our work adds to the understanding of the induction of ALP in ASCs following exposure to AH, providing important insight in how ASCs will be influenced by the ocular environment. In conclusion, increased osteogenic potential upon exposure to AH represents a potential challenge to developing ASC cell-based therapies directed at the eye.

Indexed as

Hot TemperatureAdipose TissueAlkaline PhosphataseAnalysis of VarianceAqueous HumorCells, CulturedCulture Media, Serum-FreeCytoskeletal ProteinsDose-Response Relationship, DrugEye ProteinsGlycoproteinsHumansMesenchymal Stem CellsMyocilinOsteogenesisAlkaline PhosphataseCulture Media, Serum-FreeCytoskeletal ProteinsEye ProteinsGlycoproteinsMyocilinAlkaline phosphataseAqueous humorMesenchymal stem cellsMyocilinOsteogenic potential

Identifiers

PMID25720657
PMCPMC4446238
OpenAlexW2031315167

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.