ArticlePloS one2015
An assessment of heavy ion irradiation mutagenesis for reverse genetics in wheat (Triticum aestivum L.).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Review
- Genome-wide analysis of mutations induced by carbon ion beam irradiation in cotton.Frontiers in plant science · 2023Article
- Physiological and Differential Proteomic Analysis at Seedling Stage by Induction of Heavy-Ion Beam Radiation in Wheat Seeds.Frontiers in genetics · 2022Article
- An EfficientFrontiers in plant science · 2021Article
- Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement.Plants (Basel, Switzerland) · 2019Review
- Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects.Molecular plant pathology · 2018Article
- Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpina.BMC biotechnology · 2018Article
- Transcriptomics of cereal-Fusarium graminearum interactions: what we have learned so far.Molecular plant pathology · 2018Review
- Expression ofPlant direct · 2018Article
- Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat.Scientific reports · 2017Article
- The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias.Plant biotechnology journal · 2017Article
- The Fusarium crown rot pathogen Fusarium pseudograminearum triggers a suite of transcriptional and metabolic changes in bread wheat (Triticum aestivum L.).Annals of botany · 2017Article
- Development of a High-Efficient Mutation Resource with Phenotypic Variation in Hexaploid Winter Wheat and Identification of Novel Alleles in theFrontiers in plant science · 2017Article
- The Multitalented MEDIATOR25.Frontiers in plant science · 2017Review
- Production and characterization of tearless and non-pungent onion.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetic techniques harnessing mutational approaches are powerful tools that can provide substantial insight into gene function in plants. However, as compared to diploid species, reverse genetic analyses in polyploid plants such as bread wheat can present substantial challenges associated with high levels of sequence and functional similarity amongst homoeologous loci. We previously developed a high-throughput method to identify deletions of genes within a physically mutagenized wheat population. Here we describe our efforts to combine multiple homoeologous deletions of three candidate disease susceptibility genes (TaWRKY11, TaPFT1 and TaPLDß1). We were able to produce lines featuring homozygous deletions at two of the three homoeoloci for all genes, but this was dependent on the individual mutants used in crossing. Intriguingly, despite extensive efforts, viable lines possessing homozygous deletions at all three homoeoloci could not be produced for any of the candidate genes. To investigate deletion size as a possible reason for this phenomenon, we developed an amplicon sequencing approach based on synteny to Brachypodium distachyon to assess the size of the deletions removing one candidate gene (TaPFT1) in our mutants. These analyses revealed that genomic deletions removing the locus are relatively large, resulting in the loss of multiple additional genes. The implications of this work for the use of heavy ion mutagenesis for reverse genetic analyses in wheat are discussed.
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