ArticleAnalytical chemistry2015
Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Caffeine Sodium Benzoate-Induced Macrophage M1 Polarization Promotes Endothelial Progenitor Cell Dysfunction by Inducing Mitochondrial Dysfunction Via Targeting let-7a-5p/OPA1 Axis.Applied biochemistry and biotechnology · 2026Article
- Review
- CandyCollect: at-home saliva sampling for capture of respiratory pathogens.Lab on a chip · 2022Article
- Recent advances for cancer detection and treatment by microfluidic technology, review and update.Biological procedures online · 2022Review
- The Synergy between Organ-on-a-Chip and Artificial Intelligence for the Study of NAFLD: From Basic Science to Clinical Research.Biomedicines · 2021Review
- A Modular Microscale Granuloma Model for Immune-Microenvironment Signaling StudiesFrontiers in bioengineering and biotechnology · 2020Article
- A microfluidic mammary gland coculture model using parallel 3D lumens for studying epithelial-endothelial migration in breast cancer.Biomicrofluidics · 2019Article
- Reconfigurable open microfluidics for studying the spatiotemporal dynamics of paracrine signalling.Nature biomedical engineering · 2019Article
- Engineering cell heterogeneity into organs-on-a-chip.Lab on a chip · 2018Review
- Graphene Oxide-Based Biocompatible 3D Mesh with a Tunable Porosity and Tensility for Cell Culture.ACS biomaterials science & engineering · 2018Article
- Insert-based microfluidics for 3D cell culture with analysis.Analytical and bioanalytical chemistry · 2018Article
- Design and Simulation of Microfluidic Device for Metabolite Screening and Quantitative Monitoring of Drug Uptake in Cancer Cells.Journal of electrical bioimpedance · 2018Article
- Organoids, organs-on-chips and other systems, and microbiota.Emerging topics in life sciences · 2017Article
- Microfluidic co-cultures of retinal pigment epithelial cells and vascular endothelial cells to investigate choroidal angiogenesis.Scientific reports · 2017Article
- Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment.Journal of the Royal Society, Interface · 2017Review
- Vasculature-On-A-Chip for In Vitro Disease Models.Bioengineering (Basel, Switzerland) · 2017Review
- A Critical Analysis of the AvailableJournal of ophthalmology · 2017Review
- Single cell functional analysis of multiple myeloma cell populations correlates with diffusion profiles in static microfluidic coculture systems.Biomicrofluidics · 2016Article
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Organ-on-a-Chip: New Platform for Biological Analysis.Analytical chemistry insights · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Angiogenesis (the formation of blood vessels from existing blood vessels) plays a critical role in many diseases such as cancer, benign tumors, and macular degeneration. There is a need for cell culture methods capable of dissecting the intricate regulation of angiogenesis within the microenvironment of the vasculature. We have developed a microscale cell-based assay that responds to complex pro- and antiangiogenic soluble factors with an in vitro readout for vessel formation. The power of this system over traditional techniques is that we can incorporate the whole milieu of soluble factors produced by cells in situ into one biological readout (vessel formation), even if the identity of the factors is unknown. We have currently incorporated macrophages, endothelial cells, and fibroblasts into the assay, with the potential to include additional cell types in the future. Importantly, the microfluidic platform is simple to operate and multiplex to test drugs targeting angiogenesis in a more physiologically relevant context. As a proof of concept, we tested the effect of an enzyme inhibitor (targeting matrix metalloproteinase 12) on vessel formation; the triculture microfluidic assay enabled us to capture a dose-dependent effect entirely missed in a simplified coculture assay (p < 0.0001). This result underscores the importance of cell-based assays that capture chemical cross-talk occurring between cell types. The microscale dimensions significantly reduce cell consumption compared to conventional well plate platforms, enabling the use of limited primary cells from patients in future investigations and offering the potential to screen therapeutic approaches for individual patients in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.