ArticlePloS one2015
Deep mRNA sequencing of the Tritonia diomedea brain transcriptome provides access to gene homologues for neuronal excitability, synaptic transmission and peptidergic signalling.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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17 citing papers in PubMed, 35 citations in OpenAlex.
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- Comparative Analysis of Neuropeptides in Homologous Interneurons and Prohormone Annotation in Nudipleuran Sea Slugs.Frontiers in physiology · 2021Article
- The Distribution and Possible Roles of Small Cardioactive Peptide in the NudibranchIntegrative organismal biology (Oxford, England) · 2020Article
- GABA as a Neurotransmitter in Gastropod Molluscs.The Biological bulletin · 2019Review
- Single neuron serotonin receptor subtype gene expression correlates with behaviour within and across three molluscan species.Proceedings. Biological sciences · 2018Article
- cAMP, CaACS chemical neuroscience · 2018Review
- Sequences of Circadian Clock Proteins in the Nudibranch Molluscs Hermissenda crassicornis, Melibe leonina, and Tritonia diomedea.The Biological bulletin · 2018Article
- De novo assembly, annotation, and characterization of the whole brain transcriptome of male and female Syrian hamsters.Scientific reports · 2017Article
- Comparative transcriptomics enlarges the toolkit of known developmental genes in mollusks.BMC genomics · 2016Article
- Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation.BMC genomics · 2016Article
- De novo Transcriptome Generation and Annotation for Two Korean Endemic Land Snails, Aegista chejuensis and Aegista quelpartensis, Using Illumina Paired-End Sequencing Technology.International journal of molecular sciences · 2016Article
- Article
- Identification of genes related to learning and memory in the brain transcriptome of the mollusc, Hermissenda crassicornis.Learning & memory (Cold Spring Harbor, N.Y.) · 2015Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sea slug Tritonia diomedea (Mollusca, Gastropoda, Nudibranchia), has a simple and highly accessible nervous system, making it useful for studying neuronal and synaptic mechanisms underlying behavior. Although many important contributions have been made using Tritonia, until now, a lack of genetic information has impeded exploration at the molecular level.
resultsWe performed Illumina sequencing of central nervous system mRNAs from Tritonia, generating 133.1 million 100 base pair, paired-end reads. De novo reconstruction of the RNA-Seq data yielded a total of 185,546 contigs, which partitioned into 123,154 non-redundant gene clusters (unigenes). BLAST comparison with RefSeq and Swiss-Prot protein databases, as well as mRNA data from other invertebrates (gastropod molluscs: Aplysia californica, Lymnaea stagnalis and Biomphalaria glabrata; cnidarian: Nematostella vectensis) revealed that up to 76,292 unigenes in the Tritonia transcriptome have putative homologues in other databases, 18,246 of which are below a more stringent E-value cut-off of 1x10-6. In silico prediction of secreted proteins from the Tritonia transcriptome shotgun assembly (TSA) produced a database of 579 unique sequences of secreted proteins, which also exhibited markedly higher expression levels compared to other genes in the TSA.
conclusionsOur efforts greatly expand the availability of gene sequences available for Tritonia diomedea. We were able to extract full length protein sequences for most queried genes, including those involved in electrical excitability, synaptic vesicle release and neurotransmission, thus confirming that the transcriptome will serve as a useful tool for probing the molecular correlates of behavior in this species. We also generated a neurosecretome database that will serve as a useful tool for probing peptidergic signalling systems in the Tritonia brain.
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