ArticleFrontiers in microbiology2015
Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- The anaerobic fungusmBio · 2026Article
- Characterising complex metabolic responses in an engineered, cross-feeding microbial co-culture using quantitative proteomics.New biotechnology · 2026Article
- Analysis and application of microbiota in fermentation pit muds used for Chinese strong-flavor liquor production.AMB Express · 2026Article
- Transcriptomic evidence for an energetically advantageous relationship between Syntrophomonas wolfei and Methanothrix soehngenii.Environmental microbiology reports · 2024Article
- Syntrophic microbes involved in the oxidation of short-chain fatty acids in continuous-flow anaerobic digesters treating waste activated sludge with hydrochar.Applied and environmental microbiology · 2024Article
- Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition.International journal of molecular sciences · 2023Article
- Syntrophic Propionate Oxidation: One of the Rate-Limiting Steps of Organic Matter Decomposition in Anoxic Environments.Applied and environmental microbiology · 2023Review
- Novel Long-Chain Fatty Acid (LCFA)-Degrading Bacteria and Pathways in Anaerobic Digestion Promoted by Hydrochar as Revealed by Genome-Centric Metatranscriptomics Analysis.Applied and environmental microbiology · 2022Article
- Article
- Dynamic acylome reveals metabolite driven modifications inFrontiers in microbiology · 2022Article
- The missing enzymatic link in syntrophic methane formation from fatty acids.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Catabolism and interactions of uncultured organisms shaped by eco-thermodynamics in methanogenic bioprocesses.Microbiome · 2020Article
- Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste.Microbiome · 2020Article
- Novel syntrophic bacteria in full-scale anaerobic digesters revealed by genome-centric metatranscriptomics.The ISME journal · 2020Article
- The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases.Frontiers in microbiology · 2020Article
- Article
- Syntrophus aciditrophicus uses the same enzymes in a reversible manner to degrade and synthesize aromatic and alicyclic acids.Environmental microbiology · 2019Article
- Comparative proteome analysis of propionate degradation by Syntrophobacter fumaroxidans in pure culture and in coculture with methanogens.Environmental microbiology · 2018Article
- Label-Free Proteomics of a Defined, Binary Co-culture Reveals Diversity of Competitive Responses Between Members of a Model Soil Microbial System.Microbial ecology · 2018Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial syntrophy is a vital metabolic interaction necessary for the complete oxidation of organic biomass to methane in all-anaerobic ecosystems. However, this process is thermodynamically constrained and represents an ecosystem-level metabolic bottleneck. To gain insight into the physiology of this process, a shotgun proteomics approach was used to quantify the protein landscape of the model syntrophic metabolizer, Syntrophomonas wolfei, grown axenically and syntrophically with Methanospirillum hungatei. Remarkably, the abundance of most proteins as represented by normalized spectral abundance factor (NSAF) value changed very little between the pure and coculture growth conditions. Among the most abundant proteins detected were GroEL and GroES chaperonins, a small heat shock protein, and proteins involved in electron transfer, beta-oxidation, and ATP synthesis. Several putative energy conservation enzyme systems that utilize NADH and ferredoxin were present. The abundance of an EtfAB2 and the membrane-bound iron-sulfur oxidoreductase (Swol_0698 gene product) delineated a potential conduit for electron transfer between acyl-CoA dehydrogenases and membrane redox carriers. Proteins detected only when S. wolfei was grown with M. hungatei included a zinc-dependent dehydrogenase with a GroES domain, whose gene is present in genomes in many organisms capable of syntrophy, and transcriptional regulators responsive to environmental stimuli or the physiological status of the cell. The proteomic analysis revealed an emphasis on macromolecular stability and energy metabolism by S. wolfei and presence of regulatory mechanisms responsive to external stimuli and cellular physiological status.
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