Evidence map›Paper›PMID 25717037›Full record

ArticlePlant physiology2015

Lotus japonicus clathrin heavy Chain1 is associated with Rho-Like GTPase ROP6 and involved in nodule formation.

Chao Wang, Maosheng Zhu, Liujiang Duan, Haixiang Yu, Xiaojun Chang, Li Li, Heng Kang, Yong Feng, Hui Zhu, Zonglie Hong and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Genes · 2022
    Article
  9. Review
  10. TheFrontiers in plant science · 2021
    Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chao WangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).ORCID http://orcid.org/0000-0002-5081-805X
Maosheng ZhuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Liujiang DuanState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Haixiang YuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Xiaojun ChangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Li LiState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Heng KangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Yong FengState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Hui ZhuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Zonglie HongState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.).
Zhongming ZhangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China (C.W., M.Z., L.D., H.Y., X.C., L.L., H.K., Y.F., H.Z., Z.Z.); andDepartment of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844 (Z.H.) zmzhang@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0003-1124-5989

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mechanisms underlying nodulation factor signaling downstream of the nodulation factor receptors (NFRs) have not been fully characterized. In this study, clathrin heavy chain1 (CHC1) was shown to interact with the Rho-Like GTPase ROP6, an interaction partner of NFR5 in Lotus japonicus. The CHC1 gene was found to be expressed constitutively in all plant tissues and induced in Mesorhizobium loti-infected root hairs and nodule primordia. When expressed in leaves of Nicotiana benthamiana, CHC1 and ROP6 were colocalized at the cell circumference and within cytoplasmic punctate structures. In M. loti-infected root hairs, the CHC protein was detected in cytoplasmic punctate structures near the infection pocket along the infection thread membrane and the plasma membrane of the host cells. Transgenic plants expressing the CHC1-Hub domain, a dominant negative effector of clathrin-mediated endocytosis, were found to suppress early nodulation gene expression and impair M. loti infection, resulting in reduced nodulation. Treatment with tyrphostin A23, an inhibitor of clathrin-mediated endocytosis of plasma membrane cargoes, had a similar effect on down-regulation of early nodulation genes. These findings show an important role of clathrin in the leguminous symbiosis with rhizobia.

Indexed as

Base SequenceClathrinDown-RegulationGene ExpressionGene Expression Regulation, PlantGenes, ReporterGTP PhosphohydrolasesLotusMesorhizobiumMolecular Sequence DataNicotianaPlant LeavesPlant ProteinsPlant Root NodulationPlant RootsPlants, Genetically ModifiedClathrinGTP PhosphohydrolasesPlant Proteins

Identifiers

PMID25717037
PMCPMC4378172

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.