ArticleOrigins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life2015
In situ imidazole activation of ribonucleotides for abiotic RNA oligomerization reactions.
Article in Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More.Life (Basel, Switzerland) · 2024Article
- Mineral-Mediated Oligoribonucleotide Condensation: Broadening the Scope of Prebiotic Possibilities on the Early Earth.Life (Basel, Switzerland) · 2023Article
- Thermostability, Tunability, and Tenacity of RNA as Rubbery Anionic Polymeric Materials in Nanotechnology and Nanomedicine-Specific Cancer Targeting with Undetectable Toxicity.Chemical reviews · 2021Review
- Lipid-Assisted Polymerization of Nucleotides.Life (Basel, Switzerland) · 2019Review
- Continuous nonenzymatic cross-replication of DNA strands withChemical science · 2019Article
- Exploring the Emergence of RNA Nucleosides and Nucleotides on the Early Earth.Life (Basel, Switzerland) · 2018Review
- Nucleotide Selectivity in Abiotic RNA Polymerization Reactions.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2017Article
- Nitrogenous Derivatives of Phosphorus and the Origins of Life: Plausible Prebiotic Phosphorylating Agents in Water.Life (Basel, Switzerland) · 2017Review
- Co-operation between Polymerases and Nucleotide Synthetases in the RNA World.PLoS computational biology · 2016Article
- The Effect of Limited Diffusion and Wet-Dry Cycling on Reversible Polymerization Reactions: Implications for Prebiotic Synthesis of Nucleic Acids.Life (Basel, Switzerland) · 2016Article
- Controlled supramolecular structure of guanosine monophosphate in the interlayer space of layered double hydroxide.Beilstein journal of nanotechnology · 2016Article
- Copying of RNA Sequences without Pre-Activation.Angewandte Chemie (International ed. in English) · 2015Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hypothesis that RNA played a significant role in the origin of life requires effective and efficient abiotic pathways to produce RNA oligomers. The most successful abiotic oligomerization reactions to date have utilized high-energy, modified, or pre-activated ribonucleotides to generate strands of RNA up to 50-mers in length. In spite of their success, these modifications and pre-activation reactions significantly alter the ribonucleotides in ways that are highly unlikely to have occurred on a prebiotic Earth. This research seeks to address this problem by exploring an aqueous based method for activating the canonical ribonucleotides in situ using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and imidazole. The reactions were run with and without a montmorillonite clay catalyst and compared to reactions that used ribonucleotides that were pre-activated with imidazole. The effects of pH and ribonucleotide concentration were also investigated. The results demonstrate the ability of in situ activation of ribonucleotides to generate linear RNA oligomers in solution, providing an alternative route to produce RNA for use in prebiotic Earth scenarios.
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