ArticleScientific reports2015
Optimization of the heme biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia coli.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 160 citations in OpenAlex.
- Substrate-bound structures of yeast ALA synthase reveal protein dynamics initiating heme biosynthesis.Science advances · 2026Article
- Metabolic engineering ofApplied and environmental microbiology · 2026Article
- Regulation of mitochondrial iron homeostasis in tumor cells.Molecular medicine (Cambridge, Mass.) · 2026Review
- Reactive oxygen species in the rhizosphere orchestrate the recruitment of beneficial bacteria.The EMBO journal · 2026Article
- Digital Microfluidics-Driven Cell-Free Protein Synthesis Platform Reveals Expression and Stability Determinants for Phytoglobins and Cysteine-to-Alanine Substituted Variants.Antioxidants (Basel, Switzerland) · 2025Article
- Class 1 Sugar Beet Phytoglobin Shows Strong Affinity to Glyceraldehyde-3-Phosphate Dehydrogenase and DNA In Vitro.International journal of molecular sciences · 2025Article
- Construction of a genome-engineered stable 5-aminolevulinic acid producingSynthetic and systems biotechnology · 2025Article
- Compartmentalization of heme biosynthetic pathways into yeast mitochondria enhances heme production.NPJ science of food · 2025Article
- Metabolic Engineering ofInternational journal of molecular sciences · 2024Article
- Enhanced Production of High-Value Porphyrin Compound Heme by Metabolic Engineering Modification and Mixotrophic Cultivation ofMarine drugs · 2024Article
- Optimization of Phycocyanobilin Synthesis inGenes · 2024Article
- Enhancing the biosynthesis of taxadien-5α-yl-acetate in Escherichia coli by combinatorial metabolic engineering approaches.Bioresources and bioprocessing · 2024Article
- On the Possibility of Using 5-Aminolevulinic Acid in the Light-Induced Destruction of Microorganisms.International journal of molecular sciences · 2024Review
- Promoting Heme and Phycocyanin Biosynthesis inMarine drugs · 2023Article
- Microbial Synthesis of HemeMolecules (Basel, Switzerland) · 2023Review
- Heme biosensor-guided in vivo pathway optimization and directed evolution for efficient biosynthesis of heme.Biotechnology for biofuels and bioproducts · 2023Article
- Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid.Biotechnology for biofuels and bioproducts · 2023Article
- Improved Bioproduction of the Nylon 12 Monomer by Combining the Directed Evolution of P450 and Enhancing Heme Synthesis.Molecules (Basel, Switzerland) · 2023Article
- Whole-Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine-Tuned Heme Biosynthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A Non-functional γ-Aminobutyric Acid Shunt Pathway in CyanobacteriumInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5-Aminolevulinic acid (ALA), the committed intermediate of the heme biosynthesis pathway, shows significant promise for cancer treatment. Here, we identified that in addition to hemA and hemL, hemB, hemD, hemF, hemG and hemH are also the major regulatory targets of the heme biosynthesis pathway. Interestingly, up-regulation of hemD and hemF benefited ALA accumulation whereas overexpression of hemB, hemG and hemH diminished ALA accumulation. Accordingly, by combinatorial overexpression of the hemA, hemL, hemD and hemF with different copy-number plasmids, the titer of ALA was improved to 3.25 g l(-1). Furthermore, in combination with transcriptional and enzymatic analysis, we demonstrated that ALA dehydratase (HemB) encoded by hemB is feedback inhibited by the downstream intermediate protoporphyrinogen IX. This work has great potential to be scaled-up for microbial production of ALA and provides new important insights into the regulatory mechanism of the heme biosynthesis pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.