Evidence map›Paper›PMID 25716655›Full record

ArticleJournal of applied genetics2015

Development of DArT-based PCR markers for selecting drought-tolerant spring barley.

Anna Fiust, Marcin Rapacz, Magdalena Wójcik-Jagła, Mirosław Tyrka

Abstract read
In one paragraph

Article in Journal of applied genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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  6. Association mapping of drought tolerance-related traits in barley to complement a traditional biparental QTL mapping study.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018
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  9. Magnetic Activated-ATP@FeScientific reports · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anna FiustDepartment of Plant Physiology, University of Agriculture in Kraków, ul. Podłużna 3, 30-239, Kraków, Poland.
Marcin Rapacz
Magdalena Wójcik-Jagła
Mirosław Tyrka

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The tolerance of spring barley (Hordeum vulgare L.) cultivars to spring drought is an important agronomic trait affecting crop yield and quality in Poland. Therefore, breeders require new molecular markers to select plants with lower spring drought susceptibility. With the advent of genomic selection technology, simple molecular tools may still be applicable to screen material for markers of the most important traits and in-depth genome scanning. In previous studies, diversity arrays technology (DArT)-based genetic maps were constructed for F2 populations of Polish fodder and malt barley elite breeding lines, and 15 and 18 quantitative trait loci (QTLs) related to spring drought tolerance were identified, respectively. In this paper, we show the results of a conversion of 30 DArT markers corresponding to 11 QTLs into simple sequence repeat (SSR) and sequence tagged site (STS) markers. Twenty-two polymorphic markers were obtained, including 13 DArT-based SSRs. Additionally, 31 SSR markers, located in close proximity to the DArT markers, were selected from the GrainGenes database and tested. Further analyses of 24 advanced breeding lines with different drought tolerances confirmed that five out of the 30 converted markers, as well as three out of the 31 additional SSR markers, were effective in marker-assisted selection for drought tolerance. The possible function of clones related to these markers in drought tolerance is discussed.

Indexed as

DroughtsMicrosatellite RepeatsSequence Tagged SitesBreedingChromosome MappingGenetic LinkageGenetic MarkersGenotypeHordeumPolymerase Chain ReactionQuantitative Trait LociGenetic Markers

Identifiers

PMID25716655
PMCPMC4543407

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.