ArticleJournal of clinical laboratory analysis2016
Performance Evaluation of CLIA for Treponema Pallidum Specific Antibodies Detection in Comparison with ELISA.
Article in Journal of clinical laboratory analysis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Comparison between treponemal and non-treponemal tests for syphilis detectionBiomedica : revista del Instituto Nacional de Salud · 2025Article
- Evaluation of MAGLUMI syphilis test for accurate detection of syphilis antibodies in blood donors and suspected syphilis cases.Frontiers in cellular and infection microbiology · 2025Article
- Performance characteristics of the Mindray chemiluminescence anti-Müllerian hormone assay.Journal of clinical laboratory analysis · 2021Article
- Article
- Papulosquamous eruption with ocular symptoms caused by syphilis.CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne · 2020Article
- Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Comparison of Roche Elecsys and Sysmex HISCL immunoassays for the screening of common blood-borne pathogens.Annals of translational medicine · 2019Article
- Evaluation of the Lumipulse G TP-N Chemiluminescent Immunoassay as a Syphilis Screening Test.Journal of clinical microbiology · 2017Article
- Evaluation of the HISCL Anti-Treponema pallidum Assay as a Screening Test for Syphilis.Clinical and vaccine immunology : CVI · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesIn this study we aimed to evaluate the performance effects of chemiluminescence assay (CLIA) for Treponema pallidum specific antibodies detection, and to compare T. pallidum specific antibodies detection accuracy between CLIA and ELISA with TPPA (T. pallidum particle agglutination assay) as a confirmatory test.
methodsA total of 865 samples from suspected syphilis patients and preoperative patients were included, in which T. pallidum specific antibodies were simultaneously detected by CLIA and ELISA. Among them, 457 samples were determined by TPPA.
resultsAll coefficients of variation (CVs) of ELISA in high-, median-, and low-level samples were more than 5% and the maximum CV was 54.39% in the low-level sample. CVs of CLIA in different-level samples were all below 5%. Among the three assays the Spearman correlation and Kappa coefficients were 0.771 (P ≤ 0.001) and 0.854 (P ≤ 0.001, CLIA vs. ELISA), 0.806 (P ≤ 0.001) and 0.897 (P ≤ 0.001, ELISA vs. TPPA), 0.937 (P ≤ 0.001) and 0.967 (P ≤ 0.001, CLIA vs. TPPA), respectively. The area under the receiver operating characteristic curve (AUC) of CLIA was higher than that of ELISA (0.994 vs. 0.989) with TPPA as the confirmatory test. In 18 discrepant samples the consistency rate between CLIA and TPPA was elevated compared with that between ELISA and TPPA (72.22% vs. 27.78%, P = 0.008). In gray zone, the consistency rate of CLIA with TPPA was higher than that of ELISA with TPPA (90.91% vs. 41.67%, P = 0.027).
conclusionsCompared with ELISA, CLIA is more reliable, sensitive and accurate to detect serum T. pallidum specific antibodies. In the future it may be an alternative test with higher sensitivity to ELISA.
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