ArticleDevelopment (Cambridge, England)2015
A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- In Silico Discovery of Potential Inhibitors Targeting the MEIG1-PACRG Complex for Male Contraceptive Development.Proteins · 2025Article
- Article
- Preparation of Testicular Cells for Immunofluorescence Analysis of Manchette in Elongating Spermatids.Bio-protocol · 2025Article
- Function of manchette and intra-manchette transport in spermatogenesis and male fertility.Cell communication and signaling : CCS · 2025Review
- The role of HnrnpF/H as a driver of oligoteratozoospermia.iScience · 2024Article
- GametesOmics: A Comprehensive Multi-omics Database for Exploring the Gametogenesis in Humans and Mice.Genomics, proteomics & bioinformatics · 2024Article
- Effects of preconception exposure to phthalates on mouse sperm capacitation parameters.Andrology · 2023Article
- Genome-Wide Assessment of Runs of Homozygosity by Whole-Genome Sequencing in Diverse Horse Breeds Worldwide.Genes · 2023Article
- Article
- Article
- RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling.Communications biology · 2023Article
- PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis.Development (Cambridge, England) · 2022Article
- TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse.Scientific reports · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy.Cells · 2021Article
- Exome sequencing reveals variants in known and novel candidate genes for severe sperm motility disorders.Human reproduction (Oxford, England) · 2021Article
- Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding.Structure (London, England : 1993) · 2021Article
- Motile cilia genetics and cell biology: big results from little mice.Cellular and molecular life sciences : CMLS · 2021Review
- Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization.Frontiers in cell and developmental biology · 2021Article
- Destruction or Reconstruction: A Subtle Liaison between the Proteolytic and Signaling Role of Protein Ubiquitination in Spermatogenesis.Advances in experimental medicine and biology · 2021Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
A key event in the process of spermiogenesis is the formation of the flagella, which enables sperm to reach eggs for fertilization. Yeast two-hybrid studies revealed that meiosis-expressed gene 1 (MEIG1) and Parkin co-regulated gene (PACRG) interact, and that sperm-associated antigen 16, which encodes an axoneme central apparatus protein, is also a binding partner of MEIG1. In spermatocytes of wild-type mice, MEIG1 is expressed in the whole germ cell bodies, but the protein migrates to the manchette, a unique structure at the base of elongating spermatid that directs formation of the flagella. In the elongating spermatids of wild-type mice, PACRG colocalizes with α-tubulin, a marker for the manchette, whereas this localization was not changed in the few remaining elongating spermatids of Meig1-deficient mice. In addition, MEIG1 no longer localizes to the manchette in the remaining elongating spermatids of Pacrg-deficient mice, indicating that PACRG recruits MEIG1 to the manchette. PACRG is not stable in mammalian cells, but can be stabilized by MEIG1 or by inhibition of proteasome function. SPAG16L is present in the spermatocyte cytoplasm of wild-type mice, and in the manchette of elongating spermatids, but in the Meig1 or Pacrg-deficient mice, SPAG16L no longer localizes to the manchette. By contrast, MEIG1 and PACRG are still present in the manchette of Spag16L-deficient mice, indicating that SPAG16L is a downstream partner of these two proteins. Together, our studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.