ArticlePloS one2015
Live imaging of companion cells and sieve elements in Arabidopsis leaves.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Regulatory DNA Sequences Controlling Gene Expression in Leaf Phloem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Regulatory DNA Sequences Controlling Gene Expression in Leaf Phloem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- OsDISMO1: A Novel Transporter for Molybdenum Distribution in Rice Shoots.Rice (New York, N.Y.) · 2025Article
- Developmental landscape and asymmetric gene expression in the leaf vasculature ofHorticulture research · 2025Article
- Hemolysin-like Protein of 'Microorganisms · 2025Article
- Single-cell transcriptomic analysis of flowering regulation and vernalization in Chinese cabbage shoot apex.Horticulture research · 2024Article
- PLASMODESMATA-LOCATED PROTEIN 6 regulates plasmodesmal function in Arabidopsis vasculature.The Plant cell · 2024Article
- Companion cell mediates wound-stimulated leaf-to-leaf electrical signaling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration.Quantitative plant biology · 2024Article
- Root phenotypes for improved nitrogen capture.Plant and soil · 2024Review
- Single-Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem.Genome biology · 2023Article
- The Interplay between Enucleated Sieve Elements and Companion Cells.Plants (Basel, Switzerland) · 2023Review
- Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.Journal of experimental botany · 2023Article
- Analysis of companion cell and phloem metabolism using a transcriptome-guided model of Arabidopsis metabolism.Plant physiology · 2023Article
- Dual localization of the carboxy-terminal tail of GLR3.3 in sieve element-companion cell complex.Communicative & integrative biology · 2023Article
- Single-cell transcriptome reveals dominant subgenome expression and transcriptional response to heat stress in Chinese cabbage.Genome biology · 2022Article
- Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection.The Plant cell · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phloem is a complex tissue composed of highly specialized cells with unique subcellular structures and a compact organization that is challenging to study in vivo at cellular resolution. We used confocal scanning laser microscopy and subcellular fluorescent markers in companion cells and sieve elements, for live imaging of the phloem in Arabidopsis leaves. This approach provided a simple framework for identifying phloem cell types unambiguously. It highlighted the compactness of the meshed network of organelles within companion cells. By contrast, within the sieve elements, unknown bodies were observed in association with the PP2-A1:GFP, GFP:RTM1 and RTM2:GFP markers at the cell periphery. The phloem lectin PP2-A1:GFP marker was found in the parietal ground matrix. Its location differed from that of the P-protein filaments, which were visualized with SEOR1:GFP and SEOR2:GFP. PP2-A1:GFP surrounded two types of bodies, one of which was identified as mitochondria. This location suggested that it was embedded within the sieve element clamps, specific structures that may fix the organelles to each another or to the plasma membrane in the sieve tubes. GFP:RTM1 was associated with a class of larger bodies, potentially corresponding to plastids. PP2-A1:GFP was soluble in the cytosol of immature sieve elements. The changes in its subcellular localization during differentiation provide an in vivo blueprint for monitoring this process. The subcellular features obtained with these companion cell and sieve element markers can be used as landmarks for exploring the organization and dynamics of phloem cells in vivo.
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