Comparison of the prejunctional beta-adrenoceptor stimulating actions of adrenaline and isoprenaline in the dog mesenteric vein.

1. The prejunctional beta-adrenoceptor stimulating actions of adrenaline and isoprenaline were compared by recording junction potentials from smooth muscle cells of the dog mesenteric vein. 2. The potency of adrenaline and isoprenaline on beta-adrenoceptors in the postjunctional membrane was estimated from hyperpolarization of the membrane in smooth muscle cells of the guinea-pig facial vein. In the presence of yohimbine, both agents hyperpolarized the membrane to a similar extent. 3. In the dog mesenteric vein, amplitude of the excitatory junction potential (e.j.p.) and slow depolarization was inhibited by adrenaline and potentiated by isoprenaline; the former but not the latter was accompanied by depolarization of the smooth muscle membrane. 4. In the presence of yohimbine, adrenaline inhibited the e.j.p. without depolarization of the smooth muscle membrane; the action was weaker than in the absence of yohimbine. The isoprenaline-induced potentiation of the e.j.p. was further enhanced by yohimbine. 5. It is concluded that adrenaline and isoprenaline have similar stimulating actions on postjunctional beta-adrenoceptors in the guinea-pig facial vein, but have different actions on prejunctional beta-adrenoceptors in the dog mesenteric vein; isoprenaline but not adrenaline stimulates this beta-adrenoceptor to facilitate the release of transmitter substances from perivascular noradrenergic nerves.