ArticleFrontiers in plant science2015
Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- A laser capture microdissection-based transcriptome analysis on salt glands highlights salt secretion mechanism in mangrove plant Avicennia marina.The Plant journal : for cell and molecular biology · 2026Article
- Single Nucleotide Polymorphism Charting ofFrontiers in plant science · 2020Article
- Review
- Differential expression of heat shock and floral regulatory genes in pseudocarpel initials of mantled female inflorescences from Elaeis guineensis Jacq.Plant reproduction · 2019Article
- The brown algal mode of tip growth: Keeping stress under control.PLoS biology · 2019Article
- Identification of Genes under Positive Selection Reveals Differences in Evolutionary Adaptation between Brown-Algal Species.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Laser capture microdissection (LCM) facilitates the isolation of individual cells from tissue sections, and when combined with RNA amplification techniques, it is an extremely powerful tool for examining genome-wide expression profiles in specific cell-types. LCM has been widely used to address various biological questions in both animal and plant systems, however, no attempt has been made so far to transfer LCM technology to macroalgae. Macroalgae are a collection of widespread eukaryotes living in fresh and marine water. In line with the collective effort to promote molecular investigations of macroalgal biology, here we demonstrate the feasibility of using LCM and cell-specific transcriptomics to study development of the brown alga Ectocarpus siliculosus. We describe a workflow comprising cultivation and fixation of algae on glass slides, laser microdissection, and RNA amplification. To illustrate the effectiveness of the procedure, we show qPCR data and metrics obtained from cell-specific transcriptomes generated from both upright and prostrate filaments of Ectocarpus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.