ArticleFrontiers in microbiology2015
Novel Flp pilus biogenesis-dependent natural transformation.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 56 citations in OpenAlex.
- Visualization of Type IV Pili: Linking Structural Architecture, Dynamic Function, and Translational Opportunities.Biology · 2026Review
- Cell density and single-cell heterogeneity reveal distinct competence induction dynamics in the high-GC Gram-positive Micrococcus luteus.BMC microbiology · 2026Article
- Article
- Tad pili with adaptable tips mediate contact-dependent killing during bacterial predation.Nature communications · 2025Article
- Conformational changes in the motor ATPase CpaF facilitate a rotary mechanism of Tad pilus assembly.Nature communications · 2025Article
- Adaptive laboratory evolution of Micrococcus luteus and identification of genes associated with radioresistance through genome-wide association study.Scientific reports · 2025Article
- The role of PilU in the surface behaviors ofmLife · 2025Article
- Bidirectional pilus processing in the Tad pilus system motor CpaF.Nature communications · 2024Article
- Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill.Biotechnology reports (Amsterdam, Netherlands) · 2024Article
- Tad pili contribute to the virulence and biofilm formation of virulentFrontiers in cellular and infection microbiology · 2024Article
- Assembly mechanism of a Tad secretion system secretin-pilotin complex.Nature communications · 2023Article
- Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis.Molecular genetics and genomics : MGG · 2023Article
- The Flp type IV pilus operon ofFrontiers in cellular and infection microbiology · 2022Article
- Article
- Assessing the Involvement of Selected Phenotypes ofPlants (Basel, Switzerland) · 2021Article
- Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria.Frontiers in microbiology · 2021Article
- Tad pilus-mediated twitching motility is essential for DNA uptake and survival of Liberibacters.PloS one · 2021Article
- A Type I Restriction Modification System Influences Genomic Evolution Driven by Horizontal Gene Transfer inFrontiers in microbiology · 2021Article
- Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea.Journal of bacteriology · 2020Article
- Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors inAntibiotics (Basel, Switzerland) · 2020Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural transformation has been described in bacterial species spread through nearly all major taxonomic groups. However, the current understanding of the structural components and the regulation of competence development is derived from only a few model organisms. Although natural transformation was discovered in members of the Actinobacteria (high GC Gram-positive bacteria) more than four decades ago, the structural components or the regulation of the competence system have not been studied in any representative of the entire phylum. In this report we identify a new role for a distinct type of pilus biogenesis genes (tad genes, for tight adherence), which so far have been connected only with biofilm formation, adherence and virulence traits. The tad-like genes found in the genome of Micrococcus luteus were shown to be required for genetic transformation in this actinobacterial species. We generated and analyzed individual knockout mutants for every open reading frame of the two predicted tad gene clusters as well as for a potential prepilin processing peptidase and identified the major component of the putative pili. By expressing a tagged variant of the major prepilin subunit and immunofluorescence microscopy we visualized filamentous structures extending from the cell surface. Our data indicate that the two tad gene islands complementarily contribute to the formation of a functional competence pilus in this organism. It seems likely that the involvement of tad genes in natural transformation is not unique only for M. luteus but may also prove to be the case in other representatives of the Actinobacteria, which contains important medically and biotechnologically relevant species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.